ArticleAnnals of botany2015
Nitric oxide is required for the auxin-induced activation of NADPH-dependent thioredoxin reductase and protein denitrosylation during root growth responses in arabidopsis.
Article in Annals of botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 48 citations in OpenAlex.
- Melatonin and Indole-3-Acetic Acid Synergistically Regulate Plant Growth and Stress Resistance.Cells · 2022Review
- Nitric Oxide Signaling and Its Association with Ubiquitin-Mediated Proteasomal Degradation in Plants.International journal of molecular sciences · 2022Review
- Proteomics and Interspecies Interaction Analysis Revealed Abscisic Acid Signalling to Be the Primary Driver for Oil Palm's Response against Red Palm Weevil Infestation.Plants (Basel, Switzerland) · 2021Article
- Biological Mechanisms ofAntioxidants (Basel, Switzerland) · 2021Review
- The Physiological Implications ofAntioxidants (Basel, Switzerland) · 2020Review
- Thioredoxins: Emerging Players in the Regulation of Protein S-Nitrosation in Plants.Plants (Basel, Switzerland) · 2020Review
- Article
- Hydrogen peroxide acts downstream of melatonin to induce lateral root formation.Annals of botany · 2018Article
- Melatonin and its relationship to plant hormones.Annals of botany · 2018Review
- Cross-Regulation between N Metabolism and Nitric Oxide (NO) Signaling during Plant Immunity.Frontiers in plant science · 2016Article
- Antioxidant Systems are Regulated by Nitric Oxide-Mediated Post-translational Modifications (NO-PTMs).Frontiers in plant science · 2016Review
- Unravelling how plants benefit from ROS and NO reactions, while resisting oxidative stress.Annals of botany · 2015Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
aimsAuxin is the main phytohormone controlling root development in plants. This study uses pharmacological and genetic approaches to examine the role of auxin and nitric oxide (NO) in the activation of NADPH-dependent thioredoxin reductase (NTR), and the effect that this activity has on root growth responses in Arabidopsis thaliana.
methodsArabidopsis seedlings were treated with auxin with or without the NTR inhibitors auranofin (ANF) and 1-chloro-2, 4-dinitrobenzene (DNCB). NTR activity, lateral root (LR) formation and S-nitrosothiol content were measured in roots. Protein S-nitrosylation was analysed by the biotin switch method in wild-type arabidopsis and in the double mutant ntra ntrb. KEY
resultsThe auxin-mediated induction of NTR activity is inhibited by the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (CPTIO), suggesting that NO is downstream of auxin in this regulatory pathway. The NTR inhibitors ANF and DNCB prevent auxin-mediated activation of NTR and LR formation. Moreover, ANF and DNCB also inhibit auxin-induced DR5 : : GUS and BA3 : : GUS gene expression, suggesting that the auxin signalling pathway is compromised without full NTR activity. Treatment of roots with ANF and DNCB increases total nitrosothiols (SNO) content and protein S-nitrosylation, suggesting a role of the NTR-thioredoxin (Trx)-redox system in protein denitrosylation. In agreement with these results, the level of S-nitrosylated proteins is increased in the arabidopsis double mutant ntra ntrb as compared with the wild-type.
conclusionsThe results support for the idea that NTR is involved in protein denitrosylation during auxin-mediated root development. The fact that a high NO concentration induces NTR activity suggests that a feedback mechanism to control massive and unregulated protein S-nitrosylation could be operating in plant cells.
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