ArticleBMC surgery2015
Methane-rich saline attenuates ischemia/reperfusion injury of abdominal skin flaps in rats via regulating apoptosis level.
Article in BMC surgery, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 68 citations in OpenAlex.
- Ischemia-Reperfusion Injury in Free Flaps: Molecular Mechanisms and Protective Effects of Remote Ischemic Preconditioning.Journal of cellular and molecular medicine · 2025Trial
- The contribution of probiotics to combined cellular therapy in skin wound healing in diabetic rats.Ulusal travma ve acil cerrahi dergisi = Turkish journal of trauma & emergency surgery : TJTES · 2025Article
- Beyond the Gut: Unveiling Methane's Role in Broader Physiological Systems.FASEB bioAdvances · 2025Review
- Papaverine loaded injectable and thermosensitive hydrogel system for improving survival of rat dorsal skin flaps.Journal of materials science. Materials in medicine · 2023Article
- Current Status of Experimental Animal Skin Flap Models: Ischemic Preconditioning and Molecular Factors.International journal of molecular sciences · 2022Review
- Recent advances on endogenous gasotransmitters in inflammatory dermatological disorders.Journal of advanced research · 2022Review
- Skin Microhemodynamics and Mechanisms of Its Regulation in Type 2 Diabetes Mellitus.Biophysics · 2022Article
- Improving the ischemia-reperfusion injury in vascularized composite allotransplantation: Clinical experience and experimental implications.Frontiers in immunology · 2022Review
- Methane Inhalation Protects Against Lung Ischemia-Reperfusion Injury in Rats by Regulating Pulmonary SurfactantFrontiers in physiology · 2021Article
- Lipoxin A4 protects rat skin flaps against ischemia-reperfusion injury through inhibiting cell apoptosis and inflammatory response induced by endoplasmic reticulum stress.Annals of translational medicine · 2020Article
- Methane Ameliorates Lipopolysaccharide-Induced Acute Orchitis by Anti-inflammatory, Antioxidative, and Antiapoptotic Effects via Regulation of the PK2/PKR1 Pathway.Oxidative medicine and cellular longevity · 2020Article
- Methane and Inflammation - A Review (Fight Fire with Fire).Intensive care medicine experimental · 2019Review
- Methyl-coenzyme M reductase-dependent endogenous methane enhances plant tolerance against abiotic stress and alters ABA sensitivity in Arabidopsis thaliana.Plant molecular biology · 2019Article
- Hydrogen peroxide is involved in methane-induced tomato lateral root formation.Plant cell reports · 2019Article
- Effects of bradykinin on the survival of multiterritory perforator flaps in rats.World journal of surgical oncology · 2019Article
- L-Cysteine desulfhydrase-dependent hydrogen sulfide is required for methane-induced lateral root formation.Plant molecular biology · 2019Article
- Methane Production and Bioactivity-A Link to Oxido-Reductive Stress.Frontiers in physiology · 2019Review
- Methane Alleviates Acetaminophen-Induced Liver Injury by Inhibiting Inflammation, Oxidative Stress, Endoplasmic Reticulum Stress, and Apoptosis through the Nrf2/HO-1/NQO1 Signaling Pathway.Oxidative medicine and cellular longevity · 2019Article
- Protective Effects of Methane-Rich Saline on Renal Ischemic-Reperfusion Injury in a Mouse Model.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Stromal Cell-Derived Factor-1BioMed research international · 2018Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn plastic surgery, skin damage induced by ischemia/reperfusion (I/R) is a multifactorial process that often occurs. Methane gas has been reported to be a new therapeutic gas for attenuating I/R injury. In this study, we assessed the effects of methane-rich saline (MRS) in regulating apoptosis on skin flap I/R injury.
methodsMale Sprague-Dawley rats, 6-8 weeks old, were divided randomly into three groups: one sham surgery group (SH) and two surgery groups. After undergoing 6 h of I/R management of an abdominal skin flap, surgery groups were treated with physiological saline (I/R-P) or methane-rich saline (I/R-M). On the 3rd postoperative day, a laser Doppler flowmeter was used to measure flap blood supply, and hematoxylin and eosin (H&E) staining was used to observe morphological changes. TdT-mediated dUTP-X nick end labeling (TUNEL) staining was also used to observe early apoptosis and is presented as the percentage of TUNEL-positive cells. Moreover, pASK-1, pJNK, Bcl-2 and Bax were detected by immunohistochemical technology. Caspase-3 activity was also measured to evaluate the effects of MRS.
resultsCompared to the I/R-P group, the flaps in the I/R-M group presented a larger survival area and better blood perfusion with less inflammatory infiltration and cell apoptosis, a higher expression of Bcl-2, a lower expression of pASK-1, pJNK and Bax, and a lower caspase-3 activity.
conclusionAccording to the results, MRS attenuated I/R injury by regulating apoptosis and has the potential to be applied as a new therapy for improving skin flap survival.
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