ArticleScientific reports2015
Glass is a Viable Substrate for Precision Force Microscopy of Membrane Proteins.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 61 citations in OpenAlex.
- Artifacts and Practical Issues in Atomic Force Microscopy.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Observation of Mechanical and Kinetic Distinctions between Individual Isoleucine and Arginine Residues in a Peptide Dissociating from a Model Lipid Bilayer.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Precise Fabrication of Graphite-Like Material Directly on a Biological Membrane Enabled by Ethanol Ice Resist.Nano letters · 2025Article
- Microfluidic measurement of the size and shape of lipid-anchored proteins.Biophysical journal · 2024Article
- Article
- Advantages and potential limitations of applying AFM kymograph analysis to pharmaceutically relevant membrane proteins in lipid bilayers.Scientific reports · 2023Article
- Passive and reversible area regulation of supported lipid bilayers in response to fluid flow.Biophysical journal · 2023Article
- Measuring flow-mediated protein drift across stationary supported lipid bilayers.Biophysical journal · 2023Article
- Atomic Force Microscopy Reveals Complexity Underlying General Secretory System Activity.International journal of molecular sciences · 2022Review
- A Quick and Reproducible Silanization Method by Using Plasma Activation for Hydrophobicity-Based Kinesin Single Molecule Fluorescence-Microscopy Assays.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Systematic measurements of interleaflet friction in supported bilayers.Biophysical journal · 2022Article
- Molecular characterization of alpha subunit 1 of sodium pump (ATP1A1) gene in Camelus dromedarius: its differential tissue expression potentially interprets the role in osmoregulation.Molecular biology reports · 2022Article
- Atomic force microscopy for quantitative understanding of peptide-induced lipid bilayer remodeling.Methods (San Diego, Calif.) · 2022Article
- Membrane dynamics are slowed for Alexa594-labeled membrane proteins due to substrate interactions.BBA advances · 2021Article
- Atomic Force Microscopy Reveals Membrane Protein Activity at the Single Molecule Level.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Aggregation Propensities of Herpes Simplex Virus-1 Proteins and Derived Peptides: AnACS omega · 2020Article
- Protein Translocation Activity in Surface-Supported Lipid Bilayers.Langmuir : the ACS journal of surfaces and colloids · 2019Article
- Direct visualization of theScience advances · 2019Article
- Multiple stochastic pathways in forced peptide-lipid membrane detachment.Scientific reports · 2019Article
- Single-molecule observation of nucleotide induced conformational changes in basal SecA-ATP hydrolysis.Science advances · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Though ubiquitous in optical microscopy, glass has long been overlooked as a specimen supporting surface for high resolution atomic force microscopy (AFM) investigations due to its roughness. Using bacteriorhodopsin from Halobacterium salinarum and the translocon SecYEG from Escherichia coli, we demonstrate that faithful images of 2D crystalline and non-crystalline membrane proteins in lipid bilayers can be obtained on microscope cover glass following a straight-forward cleaning procedure. Direct comparison between AFM data obtained on glass and on mica substrates show no major differences in image fidelity. Repeated association of the ATPase SecA with the cytoplasmic protrusion of SecYEG demonstrates that the translocon remains competent for binding after tens of minutes of continuous AFM imaging. This opens the door for precision long-timescale investigations of the active translocase in near-native conditions and, more generally, for integration of high resolution biological AFM with many powerful optical techniques that require non-birefringent substrates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.