ArticleApplied microbiology and biotechnology2015
Thirty-minute screening of antibiotic resistance genes in bacterial isolates with minimal sample preparation in static self-dispensing 64 and 384 assay cards.
Article in Applied microbiology and biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Contributions and Challenges of High Throughput qPCR for Determining Antimicrobial Resistance in the Environment: A Critical Review.Molecules (Basel, Switzerland) · 2019Pooled it
- Photoacoustic Flow Cytometry Using Functionalized Microspheres for Selective Detection of Bacteria.Micromachines · 2023Article
- Differentiating methicillin resistant and susceptibleFrontiers in medicine · 2023Article
- Point-of-care system for rapid real-time detection of SARS-CoV-2 virus based on commercially available Arduino platforms.Frontiers in bioengineering and biotechnology · 2022Article
- Elimination of Carryover Contamination in Real-Time Reverse Transcriptase Loop-Mediated Isothermal Amplification for Rapid Detection of the SARS-CoV-2 Virus in Point-of-Care Testing.Frontiers in cellular and infection microbiology · 2022Article
- Recent Development of Rapid Antimicrobial Susceptibility Testing Methods through Metabolic Profiling of Bacteria.Antibiotics (Basel, Switzerland) · 2021Review
- Differential DNA accessibility to polymerase enables 30-minute phenotypic β-lactam antibiotic susceptibility testing of carbapenem-resistant Enterobacteriaceae.PLoS biology · 2020Article
- A Sensitive, Specific and Simple Loop Mediated Isothermal Amplification Method for Rapid Detection ofFrontiers in microbiology · 2019Article
- Classification of Multiple DNA Dyes Based on Inhibition Effects on Real-Time Loop-Mediated Isothermal Amplification (LAMP): Prospect for Point of Care Setting.Frontiers in microbiology · 2019Article
- Rapid Phenotypic Antibiotic Susceptibility Testing of Uropathogens Using Optical Signal Analysis on the Nanowell Slide.Frontiers in microbiology · 2018Article
- Rapid antibiotic susceptibility testing from blood culture bottles with species agnostic real-time polymerase chain reaction.PloS one · 2018Article
- Rapid pathogen-specific phenotypic antibiotic susceptibility testing using digital LAMP quantification in clinical samples.Science translational medicine · 2017Article
- Quantification of microRNAs directly from body fluids using a base-stacking isothermal amplification method in a point-of-care device.Biomedical microdevices · 2017Article
- Isothermal assay targeting class 1 integrase gene for environmental surveillance of antibiotic resistance markers.Journal of environmental management · 2017Article
- Virulence factor activity relationships (VFARs): a bioinformatics perspective.Environmental science. Processes & impacts · 2017Review
- Implications of direct amplification for measuring antimicrobial resistance using point-of-care devices.Analytical methods : advancing methods and applications · 2017Article
- Identification of antibiotic resistance genes in the multidrug-resistant Acinetobacter baumannii strain, MDR-SHH02, using whole-genome sequencing.International journal of molecular medicine · 2017Article
- Isothermal amplification of environmental DNA (eDNA) for direct field-based monitoring and laboratory confirmation of Dreissena sp.PloS one · 2017Article
- Direct loop mediated isothermal amplification on filters for quantification of Dehalobacter in groundwater.Journal of microbiological methods · 2016Article
- Digital Quantification of DNA Replication and Chromosome Segregation Enables Determination of Antimicrobial Susceptibility after only 15 Minutes of Antibiotic Exposure.Angewandte Chemie (International ed. in English) · 2016Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
In a clinical setting, molecular assays such as polymerase chain reaction offer a rapid means to infer or confirm identity and therapeutic decisions. Accordingly, a number of molecular assays targeting identity and antibiotic resistance (AR) genes have been developed; however, these methods can be technically complex and relatively expensive. Herein, we describe a diagnostic concept utilizing isothermal amplification technology with non-purified heat-lysed cells and self-dispensing cards for testing multiple primers in parallel. This proof-of-concept study, performed with Staphylococcus aureus isolates and associated AR genes, was compared with culture-based susceptibility and quantitative PCR (qPCR). Results demonstrate reduced sample processing steps resulting in a turnaround time (starting from bacterial culture to ending in the antibiotic resistance gene profile) in less than 30 min. For antibiotics tested in which an associated AR gene was targeted on the Gene-Z card, 69% (18/26) of culture-based resistance events were positive for related AR genes. A comparison of loop-mediated isothermal amplification (LAMP) and qPCR assays targeting the same antibiotic resistance genes showed a 98.2% agreement in terms of presence and absence calls. Identity-based discrepancies between conventional (phenotypic) and molecular (genotypic) results were further resolved, and we were able to demonstrate higher accuracy in identification with the molecular analysis.
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