Evidence map›Paper›PMID 26227093›Full record

ArticleScientific reports2015

Stochasticity and Spatial Interaction Govern Stem Cell Differentiation Dynamics.

Quinton Smith, Evgeny Stukalin, Sravanti Kusuma, Sharon Gerecht, Sean X Sun

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
2.4field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 38 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Article
  6. Article
  7. Article
  8. Defined Microenvironments Trigger In Vitro Gastrulation in Human Pluripotent Stem Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023
    Article
  9. Review
  10. Article
  11. Review
  12. Article
  13. Review
  14. Review
  15. Cell tension and mechanical regulation of cell volume.Molecular biology of the cell · 2018
    Article
  16. Cytoskeletal tension regulates mesodermal spatial organization and subsequent vascular fate.Proceedings of the National Academy of Sciences of the United States of America · 2018
    Article
  17. Review
  18. Article
  19. Biological noise and positional effects influence cell stemness.The Journal of biological chemistry · 2018
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Quinton SmithDepartment of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218.
Evgeny Stukalin1] Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218. [2] Johns Hopkins Physical Sciences-Oncology Center, Johns Hopkins University, Baltimore, MD 21218.
Sravanti Kusuma1] Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218. [2] Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218.
Sharon Gerecht1] Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218. [2] Johns Hopkins Physical Sciences-Oncology Center, Johns Hopkins University, Baltimore, MD 21218. [3] Department of Material Science and Engineering, Johns Hopkins University, Baltimore, MD 21218.
Sean X Sun1] Department of Mechanical Engineering, Johns Hopkins University, Baltimore, MD 21218. [2] Johns Hopkins Physical Sciences-Oncology Center, Johns Hopkins University, Baltimore, MD 21218. [3] Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21218.
Johns Hopkins University · US

Funding

Engineered Stem Cell Microenvironments for controlled VasculogenesisR01HL107938 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI GERECHT, SHARON · 2011 to 2015
$2.1M
NHLBI NIH HHS R01 HL107938
6 · The paper itself

Abstract

Stem cell differentiation underlies many fundamental processes such as development, tissue growth and regeneration, as well as disease progression. Understanding how stem cell differentiation is controlled in mixed cell populations is an important step in developing quantitative models of cell population dynamics. Here we focus on quantifying the role of cell-cell interactions in determining stem cell fate. Toward this, we monitor stem cell differentiation in adherent cultures on micropatterns and collect statistical cell fate data. Results show high cell fate variability and a bimodal probability distribution of stem cell fraction on small (80-140 μm diameter) micropatterns. On larger (225-500 μm diameter) micropatterns, the variability is also high but the distribution of the stem cell fraction becomes unimodal. Using a stochastic model, we analyze the differentiation dynamics and quantitatively determine the differentiation probability as a function of stem cell fraction. Results indicate that stem cells can interact and sense cellular composition in their immediate neighborhood and adjust their differentiation probability accordingly. Blocking epithelial cadherin (E-cadherin) can diminish this cell-cell contact mediated sensing. For larger micropatterns, cell motility adds a spatial dimension to the picture. Taken together, we find stochasticity and cell-cell interactions are important factors in determining cell fate in mixed cell populations.

Indexed as

Models, BiologicalCadherinsCell CommunicationCell DifferentiationCell MovementCells, CulturedHumansStem CellsStochastic ProcessesCadherins

Identifiers

PMID26227093
PMCPMC4521170
OpenAlexW974872885

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.