Evidence map›Paper›PMID 26226617›Full record

ArticlePloS one2015

Whole-Retina Reduced Electrophysiological Activity in Mice Bearing Retina-Specific Deletion of Vesicular Acetylcholine Transporter.

Jake Bedore, Amanda C Martyn, Anson K C Li, Eric A Dolinar, Ian S McDonald, Stuart G Coupland, Vania F Prado, Marco A Prado, Kathleen A Hill

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Jake BedoreDepartment of Biology, The University of Western Ontario, London, Ontario, Canada N6A 5B7.
Amanda C MartynMolecular Medicine, Robarts Research Institute, Schulich School of Medicine & Dentistry, The University of Western Ontario, London, Ontario, Canada N6A 5B7.
Anson K C LiDepartment of Biology, The University of Western Ontario, London, Ontario, Canada N6A 5B7.
Eric A DolinarDepartment of Biology, The University of Western Ontario, London, Ontario, Canada N6A 5B7.
Ian S McDonaldDepartment of Biology, The University of Western Ontario, London, Ontario, Canada N6A 5B7.
Stuart G CouplandOphthalmology, Cellular and Molecular Medicine, University of Ottawa, Ottawa Eye Institute, Ottawa, Ontario, Canada K1H 8L6.
Vania F PradoMolecular Medicine, Robarts Research Institute, Schulich School of Medicine & Dentistry, The University of Western Ontario, London, Ontario, Canada N6A 5B7.
Marco A PradoMolecular Medicine, Robarts Research Institute, Schulich School of Medicine & Dentistry, The University of Western Ontario, London, Ontario, Canada N6A 5B7.
Kathleen A HillDepartment of Biology, The University of Western Ontario, London, Ontario, Canada N6A 5B7.
Western University · CAUniversity of Ottawa · CA

Funding

Canadian Institutes of Health Research MOP-126000Canadian Institutes of Health Research MOP-89910
6 · The paper itself

Abstract

backgroundDespite rigorous characterization of the role of acetylcholine in retinal development, long-term effects of its absence as a neurotransmitter are unknown. One of the unanswered questions is how acetylcholine contributes to the functional capacity of mature retinal circuits. The current study investigates the effects of disrupting cholinergic signalling in mice, through deletion of vesicular acetylcholine transporter (VAChT) in the developing retina, pigmented epithelium, optic nerve and optic stalk, on electrophysiology and structure of the mature retina. METHODS &

resultsA combination of electroretinography, optical coherence tomography imaging and histological evaluation assessed retinal integrity in mice bearing retina- targeted (embryonic day 12.5) deletion of VAChT (VAChTSix3-Cre-flox/flox) and littermate controls at 5 and 12 months of age. VAChTSix3-Cre-flox/flox mice did not show any gross changes in nuclear layer cellularity or synaptic layer thickness. However, VAChTSix3-Cre-flox/flox mice showed reduced electrophysiological response of the retina to light stimulus under scotopic conditions at 5 and 12 months of age, including reduced a-wave, b-wave, and oscillatory potential (OP) amplitudes and decreased OP peak power and total energy. Reduced a-wave amplitude was proportional to the reduction in b-wave amplitude and not associated with altered a-wave 10%-90% rise time or inner and outer segment thicknesses. SIGNIFICANCE: This study used a novel genetic model in the first examination of function and structure of the mature mouse retina with disruption of cholinergic signalling. Reduced amplitude across the electroretinogram wave form does not suggest dysfunction in specific retinal cell types and could reflect underlying changes in the retinal and/or extraretinal microenvironment. Our findings suggest that release of acetylcholine by VAChT is essential for the normal electrophysiological response of the mature mouse retina.

Indexed as

AcetylcholineAnimalsBlotting, WesternElectroretinographyGene DeletionMaleMiceMice, KnockoutNeurotransmitter AgentsOptic NerveReal-Time Polymerase Chain ReactionRetinaRetinal Pigment EpitheliumTomography, Optical CoherenceVesicular Acetylcholine Transport ProteinsAcetylcholineNeurotransmitter AgentsSlc18a3 protein, mouseVesicular Acetylcholine Transport Proteins

Identifiers

PMID26226617
PMCPMC4520552
OpenAlexW1621839500

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.