ArticleNature protocols2015
X-ray structure determination using low-resolution electron microscopy maps for molecular replacement.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Structures of the Insecticidal Toxin Complex Subunit XptA2 Highlight Roles for Flexible Domains.International journal of molecular sciences · 2023Article
- Mechanism of protein-primed template-independent DNA synthesis by Abi polymerases.Nucleic acids research · 2022Article
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- Challenges in solving structures from radiation-damaged tomograms of protein nanocrystals assessed by simulation.Acta crystallographica. Section D, Structural biology · 2021Article
- Using a partial atomic model from medium-resolution cryo-EM to solve a large crystal structure.Acta crystallographica. Section D, Structural biology · 2021Article
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- Structure of the Cdc48 ATPase with its ubiquitin-binding cofactor Ufd1-Npl4.Nature structural & molecular biology · 2018Article
- Article
- Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Automated structure refinement of macromolecular assemblies from cryo-EM maps using Rosetta.eLife · 2016Article
- Tackling the crystallographic structure determination of the COP9 signalosome.Acta crystallographica. Section D, Structural biology · 2016Article
- Microscopic Characterization of Membrane Transporter Function by In Silico Modeling and Simulation.Methods in enzymology · 2016Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Structures of multisubunit macromolecular machines are primarily determined either by electron microscopy (EM) or by X-ray crystallography. In many cases, a structure for a complex can be obtained at low resolution (at a coarse level of detail) with EM and at a higher resolution (with finer detail) by X-ray crystallography. The integration of these two structural techniques is becoming increasingly important for the generation of atomic models of macromolecular complexes. A low-resolution EM image can be a powerful tool for obtaining the 'phase' information that is missing from an X-ray crystallography experiment; however, integration of EM and X-ray diffraction data has been technically challenging. Here we present a step-by-step protocol that explains how low-resolution EM maps can be placed in the crystallographic unit cell by molecular replacement, and how initial phases computed from the placed EM density are extended to high resolution by averaging maps over noncrystallographic symmetry. As the resolution gap between EM and X-ray crystallography continues to narrow, the use of EM maps to help with X-ray crystal structure determination, as described in this protocol, will become increasingly effective.
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