ArticleJournal of clinical microbiology2015
Direct Detection of Erythromycin-Resistant Bordetella pertussis in Clinical Specimens by PCR.
Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.
- Antimicrobial resistance inEpidemiology and infection · 2026Pooled it
- Review
- The potential of wastewater monitoring as a novel surveillance tool for early warning ofEmerging microbes & infections · 2025Article
- Microbiological treatment failure associated with macrolide-resistantASM case reports · 2025Article
- Macrolide-resistantEuro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2024Article
- StrengtheningJournal of clinical microbiology · 2024Article
- Genotyping and macrolide-resistant mutation of Bordetella pertussis in East and South-East Asia.Journal of global antimicrobial resistance · 2022Article
- Macrolide Resistance inAntibiotics (Basel, Switzerland) · 2022Review
- Genomic and transcriptomic variation in Bordetella spp. following induction of erythromycin resistance.The Journal of antimicrobial chemotherapy · 2022Article
- Macrolide susceptibility and molecular characteristics ofThe Journal of international medical research · 2022Article
- Decline of serologic immunity to diphtheria, tetanus and pertussis with age suggested a full life vaccination in mainland China.Human vaccines & immunotherapeutics · 2021Article
- The global prevalence ptxP3 lineage of Bordetella pertussis was rare in young children with the co-purified aPV vaccination: a 5 years retrospective study.BMC infectious diseases · 2020Article
- Genomic epidemiology of erythromycin-resistant Bordetella pertussis in China.Emerging microbes & infections · 2019Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Resistance of Bordetella pertussis to erythromycin has been increasingly reported. We developed an allele-specific PCR method for rapid detection of erythromycin-resistant B. pertussis directly from nasopharyngeal (NP) swab samples submitted for diagnostic PCR. Based on the proven association of erythromycin resistance with the A2047G mutation in the 23S rRNA of B. pertussis, four primers, two of which were designed to be specific for either the wild-type or the mutant allele, were used in two different versions of the allele-specific PCR assay. The methods were verified with results obtained by PCR-based sequencing of 16 recent B. pertussis isolates and 100 NP swab samples submitted for diagnostic PCR. The detection limits of the two PCR assays ranged from 10 to 100 fg per reaction for both erythromycin-susceptible and -resistant B. pertussis. Two amplified fragments of each PCR, of 286 and 112 bp, respectively, were obtained from a mutant allele of the isolates and/or NP swab samples containing B. pertussis DNAs. For the wild-type allele, only a 286-bp fragment was visible when the allele-specific PCR assay 1 was performed. No amplification was found when a number of non-Bordetella bacterial pathogens and NP swab samples that did not contain the DNAs of B. pertussis were examined. This assay can serve as an alternative for PCR-based sequencing, especially for local laboratories in resource-poor countries.
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Registered trials
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