ArticlePlant physiology2015
A Single Amino Acid Substitution in an ORANGE Protein Promotes Carotenoid Overaccumulation in Arabidopsis.
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Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 109 citations in OpenAlex.
- ORANGE: a tale of two pigments and two organelles.Plant cell reports · 2026Review
- The mitochondrial DnaJA protein LMA1 regulates reactive oxygen species homeostasis and anthocyanin biosynthesis inPlant diversity · 2026Article
- Boosting β-carotene in rice and wheat grains through seed-specific expression of a modified wheat or gene.Transgenic research · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Chloroplast budding mediates β-carotene transport for early stage astaxanthin hyperaccumulation in microalgae.Plant physiology · 2025Article
- Arabidopsis phytyl ester synthases PES1 and PES2 moonlighted as xanthophyll acyltransferases in plants.Planta · 2025Article
- ORANGE family proteins: multifunctional chaperones shaping plant carotenoid level, plastid development, stress tolerance, and more.Molecular horticulture · 2025Review
- Genome-Wide Association Study of Sweet Potato Storage Root Traits Using GWASpoly, a Gene Dosage-Sensitive Model.International journal of molecular sciences · 2024Article
- Nudix hydrolase 23 post-translationally regulates carotenoid biosynthesis in plants.The Plant cell · 2024Article
- Creating Climate-Resilient Crops by Increasing Drought, Heat, and Salt Tolerance.Plants (Basel, Switzerland) · 2024Review
- Roles of Two Phytoene Synthases and Orange Protein in Carotenoid Metabolism of the β-Carotene-AccumulatingMicrobiology spectrum · 2023Article
- Biochemical Characterization of Orange-Colored Rice Calli Induced by Target Mutagenesis ofPlants (Basel, Switzerland) · 2022Article
- Plastid diversity and chromoplast biogenesis in differently coloured carrots: role of the DcOR3Planta · 2022Article
- Mapping and Validation ofInternational journal of molecular sciences · 2022Article
- Plant carotenoids: recent advances and future perspectives.Molecular horticulture · 2022Review
- Characterization of cassava ORANGE proteins and their capability to increase provitamin A carotenoids accumulation.PloS one · 2022Article
- Phytoene Synthase: The Key Rate-Limiting Enzyme of Carotenoid Biosynthesis in Plants.Frontiers in plant science · 2022Review
- Metabolic Engineering of Crocin Biosynthesis inFrontiers in plant science · 2022Article
- Overexpression of Orange Gene (Genes · 2021Article
- Multi-strategy engineering greatly enhances provitamin A carotenoid accumulation and stability in Arabidopsis seeds.aBIOTECH · 2021Article
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Authors and funding
19 authors at 6 institutions in 5 countries.
Hui YuanRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Katherine OwsianyRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
T E SheejaRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Xiangjun ZhouRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Caroline RodriguezRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Yongxi LiRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Ralf WelschRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Noam ChayutRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Yong YangRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Theodore W ThannhauserRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Mandayam V ParthasarathyRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Qiang XuRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Xiuxin DengRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Zhangjun FeiRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Ari SchafferRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Nurit KatzirRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Joseph BurgerRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Yaakov TadmorRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.).
Li LiRobert W. Holley Center for Agriculture and Health, U.S. Department of Agriculture-Agricultural Research Service (H.Y., X.Z., Y.Y., T.W.T., L.L.), Plant Breeding and Genetics Section, School of Integrative Plant Science (H.Y., K.O., T.E.S., X.Z., C.R., L.L.), Plant Biology Section, School of Integrative Plant Science (M.V.P.), and Boyce Thompson Institute for Plant Research (Z.F.), Cornell University, Ithaca, New York 14853;Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan 430070, People's Republic of China (Y.L., Q.X., X.D.);University of Freiburg, Faculty of Biology II, D79104 Freiburg, Germany (R.W.); andNewe Ya'ar Research Center, Agricultural Research Organization, Ramat Yishay 30095, Israel (N.C., A.S., N.K., J.B., Y.T.) ll37@cornell.edu.
Agricultural Research Organization · ILRobert W. Holley Center for Agriculture & Health · USHuazhong Agricultural University · CNInstitute of Plant Genetics, Polish Academy of Sciences · PLCornell University · USUniversity of Freiburg · DE
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carotenoids are crucial for plant growth and human health. The finding of ORANGE (OR) protein as a pivotal regulator of carotenogenesis offers a unique opportunity to comprehensively understand the regulatory mechanisms of carotenoid accumulation and develop crops with enhanced nutritional quality. Here, we demonstrated that alteration of a single amino acid in a wild-type OR greatly enhanced its ability to promote carotenoid accumulation. Whereas overexpression of OR from Arabidopsis (Arabidopsis thaliana; AtOR) or from the agronomically important crop sorghum (Sorghum bicolor; SbOR) increased carotenoid levels up to 2-fold, expression of AtOR(His) (R90H) or SbOR(His) (R104H) variants dramatically enhanced carotenoid accumulation by up to 7-fold in the Arabidopsis calli. Moreover, we found that AtOR(Ala) (R90A) functioned similarly to AtOR(His) to promote carotenoid overproduction. Neither AtOR nor AtOR(His) greatly affected carotenogenic gene expression. AtOR(His) exhibited similar interactions with phytoene synthase (PSY) as AtOR in posttranscriptionally regulating PSY protein abundance. AtOR(His) triggered biogenesis of membranous chromoplasts in the Arabidopsis calli, which shared structures similar to chromoplasts found in the curd of the orange cauliflower (Brassica oleracea) mutant. By contrast, AtOR did not cause plastid-type changes in comparison with the controls, but produced plastids containing larger and electron-dense plastoglobuli. The unique ability of AtOR(His) in mediating chromoplast biogenesis is responsible for its induced carotenoid overproduction. Our study demonstrates OR(His/Ala) as powerful tools for carotenoid enrichment in plants, and provides insights into the mechanisms underlying OR(His)-regulated carotenoid accumulation.
Indexed as
Amino Acid SubstitutionAmino Acid SequenceArabidopsisArabidopsis ProteinsBiosynthetic PathwaysCarotenoidsGene Expression Regulation, PlantHSP40 Heat-Shock ProteinsMolecular Sequence DataPlants, Genetically ModifiedPlastidsProtein TransportSequence AlignmentArabidopsis ProteinsCarotenoidsHSP40 Heat-Shock ProteinsORANGE protein, Arabidopsis
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