ArticlePlant physiology2015
Populus euphratica APYRASE2 Enhances Cold Tolerance by Modulating Vesicular Trafficking and Extracellular ATP in Arabidopsis Plants.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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61 citing papers in PubMed, 112 citations in OpenAlex.
- Elucidation of the genetic architecture stabilizing the heading time in barley (Hordeum vulgare L.).Planta · 2026Article
- Evolution and stress-responsive regulation of the apyrase gene family in Chinese cabbage (Frontiers in plant science · 2026Article
- Apyrase in horticultural crops: insights into growth, stress adaptation and quality regulation.Molecular horticulture · 2025Review
- Uridine Kinase-like Protein (GhUKL4) Positively Regulates Resistance to Verticillium Wilt in Cotton.Genes · 2025Article
- Extracellular ATP: an emerging multifaceted regulator of plant fitness.Plant biotechnology journal · 2025Review
- Genome-Wide Analysis of Soybean Apyrase Gene Family and Functional Characterization of GmAPY1-4 Responses to Aluminum Stress.International journal of molecular sciences · 2025Article
- Extracellular AMP Inhibits Pollen Tube Growth inPlants (Basel, Switzerland) · 2024Article
- Is ATP a signaling regulator for postharvest chilling tolerance in fruits?Horticulture research · 2024Article
- Alfin-like (AL) transcription factor family inBiotechnology reports (Amsterdam, Netherlands) · 2024Article
- Genome-wide identification and analyses ofMolecular breeding : new strategies in plant improvement · 2024Article
- Physiological and transcriptomic comparisons shed light on the cold stress response mechanisms of Dendrobium spp.BMC plant biology · 2024Article
- Growth regulation by apyrases: Insights from altering their expression level in different organisms.Plant physiology · 2024Review
- Seasonal dynamics of cell-to-cell transport in angiosperm wood.Journal of experimental botany · 2024Article
- Pectin demethylation-mediated cell wall NaTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Extracellular Self- and Non-Self DNA Involved in Damage Recognition in the Mistletoe Parasitism of Mesquite Trees.International journal of molecular sciences · 2023Article
- Article
- The Raf-like MAPKKK INTEGRIN-LINKED KINASE 5 regulates purinergic receptor-mediated innate immunity in Arabidopsis.The Plant cell · 2023Article
- Comparative transcriptomics and co-expression networks reveal cultivar-specific molecular signatures associated with reproductive-stage cold stress in rice.Plant cell reports · 2023Article
- Genome-Wide Investigation of Apyrase (APY) Genes in Peanut (International journal of molecular sciences · 2023Article
- Article
1 more citing papers are in PubMed but not listed here.
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15 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Apyrase and extracellular ATP play crucial roles in mediating plant growth and defense responses. In the cold-tolerant poplar, Populus euphratica, low temperatures up-regulate APYRASE2 (PeAPY2) expression in callus cells. We investigated the biochemical characteristics of PeAPY2 and its role in cold tolerance. We found that PeAPY2 predominantly localized to the plasma membrane, but punctate signals also appeared in the endoplasmic reticulum and Golgi apparatus. PeAPY2 exhibited broad substrate specificity, but it most efficiently hydrolyzed purine nucleotides, particularly ATP. PeAPY2 preferred Mg(2+) as a cofactor, and it was insensitive to various, specific ATPase inhibitors. When PeAPY2 was ectopically expressed in Arabidopsis (Arabidopsis thaliana), cold tolerance was enhanced, based on root growth measurements and survival rates. Moreover, under cold stress, PeAPY2-transgenic plants maintained plasma membrane integrity and showed reduced cold-elicited electrolyte leakage compared with wild-type plants. These responses probably resulted from efficient plasma membrane repair via vesicular trafficking. Indeed, transgenic plants showed accelerated endocytosis and exocytosis during cold stress and recovery. We found that low doses of extracellular ATP accelerated vesicular trafficking, but high extracellular ATP inhibited trafficking and reduced cell viability. Cold stress caused significant increases in root medium extracellular ATP. However, under these conditions, PeAPY2-transgenic lines showed greater control of extracellular ATP levels than wild-type plants. We conclude that Arabidopsis plants that overexpressed PeAPY2 could increase membrane repair by accelerating vesicular trafficking and hydrolyzing extracellular ATP to avoid excessive, cold-elicited ATP accumulation in the root medium and, thus, reduced ATP-induced inhibition of vesicular trafficking.
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