ArticleAmerican journal of physiology. Renal physiology2015
NBCe1 expression is required for normal renal ammonia metabolism.
Article in American journal of physiology. Renal physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 35 citations in OpenAlex.
- Amyloid Precursor Protein Processing Links Female Urgency Urinary Incontinence with Alzheimer's Disease: Implications for Treatment.International journal of molecular sciences · 2026Article
- The proximal tubule through an NBCe1-dependent mechanism regulates collecting duct phenotypic and remodeling responses to acidosis.American journal of physiology. Renal physiology · 2023Article
- Smooth muscle AKG/OXGR1 signaling regulates epididymal fluid acid-base balance and sperm maturation.Life metabolism · 2022Article
- Acid-base effects of combined renal deletion of NBCe1-A and NBCe1-B.American journal of physiology. Renal physiology · 2022Article
- Role of the renal androgen receptor in sex differences in ammonia metabolism.American journal of physiology. Renal physiology · 2021Article
- Expression of lactate dehydrogenase A and B isoforms in the mouse kidney.American journal of physiology. Renal physiology · 2021Article
- Sex differences in renal ammonia metabolism.American journal of physiology. Renal physiology · 2021Article
- Acetazolamide causes renalAmerican journal of physiology. Renal physiology · 2020Article
- Testosterone modulates renal ammonia metabolism.American journal of physiology. Renal physiology · 2020Article
- Differences in acidosis-stimulated renal ammonia metabolism in the male and female kidney.American journal of physiology. Renal physiology · 2019Article
- Regulation of renal NaDC1 expression and citrate excretion by NBCe1-A.American journal of physiology. Renal physiology · 2019Article
- Emerging Features of Ammonia Metabolism and Transport in Acid-Base Balance.Seminars in nephrology · 2019Review
- Acid-base regulation in the renal proximal tubules: using novel pH sensors to maintain homeostasis.American journal of physiology. Renal physiology · 2018Review
- Expression of the B splice variant of NBCe1 (SLC4A4) in the mouse kidney.American journal of physiology. Renal physiology · 2018Article
- Differences in renal ammonia metabolism in male and female kidney.American journal of physiology. Renal physiology · 2018Article
- Renal Tubular Acidosis: HAdvances in chronic kidney disease · 2018Review
- Mechanism of Hyperkalemia-Induced Metabolic Acidosis.Journal of the American Society of Nephrology : JASN · 2018Article
- NBCe1-A Regulates Proximal Tubule Ammonia Metabolism under Basal Conditions and in Response to Metabolic Acidosis.Journal of the American Society of Nephrology : JASN · 2018Article
- Proximal tubule glutamine synthetase expression is necessary for the normal response to dietary protein restriction.American journal of physiology. Renal physiology · 2017Article
- Ammonia Transporters and Their Role in Acid-Base Balance.Physiological reviews · 2017Review
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Authors and funding
6 authors at 3 institutions in 1 country.
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Abstract
The mechanisms regulating proximal tubule ammonia metabolism are incompletely understood. The present study addressed the role of the proximal tubule basolateral electrogenic Na(+)-coupled bicarbonate cotransporter (NBCe1; Slc4a4) in renal ammonia metabolism. We used mice with heterozygous and homozygous NBCe1 gene deletion and compared these mice with their wild-type littermates. Because homozygous NBCe1 gene deletion causes 100% mortality before day 25, we studied mice at day 8 (±1 day). Both heterozygous and homozygous gene deletion caused a gene dose-related decrease in serum bicarbonate. The ability to lower urinary pH was intact, and even accentuated, with NBCe1 deletion. However, in contrast to the well-known effect of metabolic acidosis to increase urinary ammonia excretion, NBCe1 deletion caused a gene dose-related decrease in ammonia excretion. There was no identifiable change in proximal tubule structure by light microscopy. Examination of proteins involved in renal ammonia metabolism showed decreased expression of phosphate-dependent glutaminase and phosphoenolpyruvate carboxykinase, key enzymes in proximal tubule ammonia generation, and increased expression of glutamine synthetase, which recycles intrarenal ammonia and regenerates glutamine. Expression of key proteins involved in ammonia transport outside of the proximal tubule (rhesus B glycoprotein and rhesus C glycoprotein) was not significantly changed by NBCe1 deletion. We conclude from these findings that NBCe1 expression is necessary for normal proximal tubule ammonia metabolism.
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