ArticleRice (New York, N.Y.)2014
A non-radioactive method for small RNA detection by northern blotting.
Article in Rice (New York, N.Y.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- RNA Labeling for Measuring RNA Endonucleolytic Cleavage.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Article
- Small Nucleolar RNAs: Biological Functions and Diseases.MedComm · 2025Review
- Nanopore-based detection of periodontitis biomarker miR31 in saliva samples.Electrophoresis · 2024Article
- Characterization of Argonaute-containing protein complexes in Leishmania-infected human macrophages.PloS one · 2024Article
- An electrochemical determination strategy for miRNA based on bimetallic nanozyme and toehold-mediated DNA replacement procedure.Mikrochimica acta · 2023Article
- The key micronutrient copper orchestrates broad-spectrum virus resistance in rice.Science advances · 2022Article
- Considerations and Suggestions for the Reliable Analysis of miRNA in Plasma Using qRT-PCR.Genes · 2022Review
- A high-throughput screening RT-qPCR assay for quantifying surrogate markers of immunity from PBMCs.Frontiers in immunology · 2022Article
- Review
- A Comprehensive Analysis of Northern versus Liquid Hybridization Assays for mRNAs, Small RNAs, and miRNAs Using a Non-Radiolabeled Approach.Current issues in molecular biology · 2021Article
- Structural-profiling of low molecular weight RNAs by nanopore trapping/translocation using Mycobacterium smegmatis porin A.Nature communications · 2021Article
- Rcl1 depletion impairs 18S pre-rRNA processing at the A1-site and up-regulates a cohort of ribosome biogenesis genes in zebrafish.Nucleic acids research · 2021Article
- Non-coding RNAs in cancer: platforms and strategies for investigating the genomic "dark matter".Journal of experimental & clinical cancer research : CR · 2020Review
- MicroRNA-like milR236, regulated by transcription factor MoMsn2, targets histone acetyltransferase MoHat1 to play a role in appressorium formation and virulence of the rice blast fungus Magnaporthe oryzae.Fungal genetics and biology : FG & B · 2020Article
- Near-infrared fluorescent northern blot.RNA (New York, N.Y.) · 2018Article
- tRNA fragments (tRFs) guide Ago to regulate gene expression post-transcriptionally in a Dicer-independent manner.RNA (New York, N.Y.) · 2018Article
- Resistance to CymMV and ORSV in artificial microRNA transgenic Nicotiana benthamiana plants.Scientific reports · 2018Article
- Comparative genome-wide analysis of extracellular small RNAs from the mucormycosis pathogen Rhizopus delemar.Scientific reports · 2018Article
- Detection and Verification of Mammalian Mirtrons by Northern Blotting.Methods in molecular biology (Clifton, N.J.) · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSmall non-coding RNAs are essential regulators of gene expression at the transcriptional and posttranscriptional levels. High-throughput sequencing has revealed thousands of predicted small RNAs; however, only a few of these have been well characterized. Northern blotting is the most convincing method for small RNA validation.
findingsIn this study, we improved the Northern blot method by using biotin-labeled probes. miRNAs and siRNAs derived from both Arabidopsis thaliana and Oryza sativa were investigated. The results suggest that this improved method is sensitive and efficient, with approximately 5 μg of total RNA being sufficient for detection. Furthermore, long-term storage of probes labeled in this manner is more convenient, less contaminative and degradative compared with traditional probes.
conclusionsThis protocol is an alternative strategy for small RNA detection and represents an efficient means of researching small RNAs.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.