ArticleClinical oral investigations2016
Osteoblast differentiation is enhanced by a nano-to-micro hybrid titanium surface created by Yb:YAG laser irradiation.
Article in Clinical oral investigations, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 44 citations in OpenAlex.
- Surface modifications and coatings to improve osseointegration and antimicrobial activity on titanium surfaces: A statistical review over the last decade.Journal of orthopaedics · 2025Review
- Synthesis, Characterization, and Osteogenic Ability of Fibrillar Polycaprolactone Scaffolds Containing Hydroxyapatite Nanoparticles.ACS applied materials & interfaces · 2025Article
- Early Peri-Implant Bone Healing on Laser-Modified Surfaces with and without Hydroxyapatite Coating: An In Vivo Study.Biology · 2024Article
- Infrared diode laser enhances human periodontal ligament stem cells behaviour on titanium dental implants.Scientific reports · 2024Article
- Synergistic Enhancement of Protein Recruitment and Retention via Implant Surface Microtopography and Superhydrophilicity in a Computational Fluid Dynamics Model.International journal of molecular sciences · 2023Article
- Laser-Modified Ti Surface Improves Paracrine Osteogenesis by Modulating the Expression of DKK1 in Osteoblasts.Journal of functional biomaterials · 2023Article
- Implant-bone-interface: Reviewing the impact of titanium surface modifications on osteogenic processes in vitro and in vivo.Bioengineering & translational medicine · 2022Review
- Evaluation of Preosteoblast MC3T3-E1 Cells Cultured on a Microporous Titanium Membrane Fabricated Using a Precise Mechanical Punching Process.Materials (Basel, Switzerland) · 2020Article
- Photo and Plasma Activation of Dental Implant Titanium Surfaces. A Systematic Review with Meta-Analysis of Pre-Clinical Studies.Journal of clinical medicine · 2020Review
- Preparation of Superhydrophobic Surface on Titanium Alloy via Micro-milling, Anodic Oxidation and Fluorination.Micromachines · 2020Article
- Biological characterization of surface-treated dental implant materials in contact with mammalian host and bacterial cells: titaniumRSC advances · 2019Article
- Ginsenoside Rb1 prevents steroid‑induced avascular necrosis of the femoral head through the bone morphogenetic protein‑2 and vascular endothelial growth factor pathway.Molecular medicine reports · 2019Article
- Proliferation of Osteoblasts on Laser-Modified Nanostructured Titanium Surfaces.Materials (Basel, Switzerland) · 2018Article
- Photofunctionalization and non-thermal plasma activation of titanium surfaces.Clinical oral investigations · 2018Article
- Osteoblastic cell response on high-rough titanium coatings by cold spray.Journal of materials science. Materials in medicine · 2018Article
- Enhancing effects of basic fibroblast growth factor and fibronectin on osteoblast adhesion to bone scaffolds for bone tissue engineering through extracellular matrix-integrin pathway.Experimental and therapeutic medicine · 2017Article
- Laser-modified titanium surfaces enhance the osteogenic differentiation of human mesenchymal stem cells.Stem cell research & therapy · 2017Article
- In vitro biological outcome of laser application for modification or processing of titanium dental implants.Lasers in medical science · 2017Review
- The response of osteoblastic MC3T3-E1 cells to micro- and nano-textured, hydrophilic and bioactive titanium surfaces.Journal of materials science. Materials in medicine · 2016Article
- Nanoscale Surface Modifications of Orthopaedic Implants: State of the Art and Perspectives.The open orthopaedics journal · 2016Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe aim of this study was to analyze the capacity of a new modified laser surface to stimulate calvarial osteoblasts isolated from neonatal mouse bones to differentiate and form mineralized nodules.
methodsTitanium discs were subjectezd or not to laser irradiation according to specific parameters and characterized. Osteoblasts isolated from neonatal mouse calvaria were cultured over the discs, and the capacity of these cells to proliferate (MTT assay), form mineralized nodules (Alizarin red assay), and enhance alkaline phosphatase activity (ALPase activity) was analyzed. Real-time PCR was used for quantification of gene expression.
resultsLaser-irradiated titanium discs (L) presented a rough nano-to-micrometric oxidized surface contrasting with the smooth pattern on polished discs (P). The Ra on the micrometric level increased from 0.32 ± 0.01 μm on P surfaces to 10.57 ± 0.39 μm on L surfaces. When compared with P, L promoted changes in osteoblast morphology, increased mineralized nodule formation in osteoblasts cultured on the surfaces for 14 days, and enhanced ALPase activity at days 7 and 14. Transcription factors triggering osteoblast differentiation (Runx2 and Sp7) and genes encoding the bone extracellular matrix proteins collagen type-1 (Col1a1), osteopontin (Spp1), and osteocalcin (Bglap) were upregulated in cells on L surfaces compared with those on P surfaces at days 1-14.
conclusionLaser treatment of titanium surfaces created a rough surface that stimulated osteoblast differentiation. CLINICAL RELEVANCE: Laser treatment of titanium generates a reproducible and efficient surface triggering osteoblast differentiation that can be of importance for osteointegration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.