ArticleProbiotics and antimicrobial proteins2015
Feasibility of Genome-Wide Screening for Biosafety Assessment of Probiotics: A Case Study of Lactobacillus helveticus MTCC 5463.
Article in Probiotics and antimicrobial proteins, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Current Concepts in Probiotic Safety and Efficacy.Nutrients · 2026Review
- Probiotic Potential of Traditional and Emerging Microbial Strains in Functional Foods: From Characterization to Applications and Health Benefits.Microorganisms · 2025Review
- Safety and Probiotics Evaluation of Bifidobacterial Genomes Isolated from Probiotic Products.Current microbiology · 2025Article
- Complete Genome Sequence and Probiotic Properties of Pediococcus acidilactici CLP03 Isolated from Healthy Felis catus.Probiotics and antimicrobial proteins · 2025Article
- Developing Gut-Healthy Strains for Pets: Probiotic Potential and Genomic Insights of Canine-DerivedMicroorganisms · 2025Article
- Isolation, probiotic properties, and whole-genome analysis ofFrontiers in microbiology · 2025Article
- Genomic Characterization ofGenes · 2024Article
- Isolation and Characterisation ofFoods (Basel, Switzerland) · 2024Article
- Recent and Advanced DNA-Based Technologies for the Authentication of Probiotic, Protected Designation of Origin (PDO) and Protected Geographical Indication (PGI) Fermented Foods and Beverages.Foods (Basel, Switzerland) · 2023Review
- Probiogenomic Analysis of Lactiplantibacillus sp. LM14-2 from Fermented Mussel (Hoi-dong), and Evaluation of its Cholesterol-lowering and Immunomodulation Effects.Probiotics and antimicrobial proteins · 2023Article
- Safety Evaluation of Lactobacillus delbrueckii subsp. lactis CIDCA 133: a Health-Promoting Bacteria.Probiotics and antimicrobial proteins · 2022Article
- In Silico Genomic and Metabolic Atlas ofMicroorganisms · 2022Article
- Potential Probiotic Strains From Milk and Water Kefir Grains in Singapore-Use for Defense Against Enteric Bacterial Pathogens.Frontiers in microbiology · 2022Article
- Complete genome sequence and comparative analysis of two potential probiotics Bacillus subtilis isolated from honey and honeybee microbiomes.Journal, genetic engineering & biotechnology · 2020Article
- Safety Assessment of a Nham Starter Culture Lactobacillus plantarum BCC9546 via Whole-genome Analysis.Scientific reports · 2020Article
- Genomic Comparison ofFrontiers in microbiology · 2019Article
- Safety Assessment of Lactobacillus helveticus KLDS1.8701 Based on Whole Genome Sequencing and Oral Toxicity Studies.Toxins · 2017Article
- Protective Effect of Lactobacillus casei on DMH-Induced Colon Carcinogenesis in Mice.Probiotics and antimicrobial proteins · 2017Article
- Regulatory and Safety Requirements for Food Cultures.Microorganisms · 2017Review
- In silico analysis of protein toxin and bacteriocins from Lactobacillus paracasei SD1 genome and available online databases.PloS one · 2017Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent years have witnessed an explosion in genome sequencing of probiotic strains for accurate identification and characterization. Regulatory bodies are emphasizing on the need for performing phase I safety studies for probiotics. The main hypothesis of this study was to explore the feasibility of using genome databases for safety screening of strains. In this study, we attempted to develop a framework for the safety assessment of a potential probiotic strain, Lactobacillus helveticus MTCC 5463 based on genome mining for genes associated with antibiotic resistance, production of harmful metabolites, and virulence. The sequencing of MTCC 5463 was performed using GS-FLX Titanium reagents. Genes coding for antibiotic resistance and virulence were identified using Antibiotic Resistance Genes Database and Virulence Factors Database. Results indicated that MTCC 5463 carried antibiotic resistance genes associated with beta-lactam and fluoroquinolone. There is no threat of transfer of these genes to host gut commensals because the genes are not plasmid encoded. The presence of genes for adhesion, biofilm, surface proteins, and stress-related proteins provides robustness to the strain. The presence of hemolysin gene in the genome revealed a theoretical risk of virulence. The results of in silico analysis complemented the in vitro studies and human clinical trials, confirming the safety of the probiotic strain. We propose that the safety assessment of probiotic strains administered live at high doses using a genome-wide screening could be an effective and time-saving tool for identifying prognostic biomarkers of biosafety.
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