ArticlePloS one2015
Arabidopsis Raf-Like Mitogen-Activated Protein Kinase Kinase Kinase Gene Raf43 Is Required for Tolerance to Multiple Abiotic Stresses.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 39 citations in OpenAlex.
- Genome-Wide Identification ofPlants (Basel, Switzerland) · 2026Article
- Comparative transcriptomic and biochemical analyses reveal candidate regulatory genes associated with early drought response signatures in contrasting rapeseed (Brassica napus) cultivars.Scientific reports · 2025Article
- Transcriptomic Identification of Long Noncoding RNAs Modulating MPK3/MPK6-Centered Immune Networks in Arabidopsis.International journal of molecular sciences · 2025Article
- Genomic introgression underlies environmental adaptation in three species of Chinese wingnuts,Plant diversity · 2025Article
- MAPKKKs in Plants: Multidimensional Regulators of Plant Growth and Stress Responses.International journal of molecular sciences · 2023Review
- Genome-wide identification and expression analysis of Raf-like kinase gene family in pepper (Plant signaling & behavior · 2022Article
- Functional analysis of mitogen-activated protein kinases (MAPKs) in potato under biotic and abiotic stress.Molecular breeding : new strategies in plant improvement · 2022Review
- Transcriptome and Physiological Analyses of a Navel Orange Mutant with Improved Drought Tolerance and Water Use Efficiency Caused by Increases of Cuticular Wax Accumulation and ROS Scavenging Capacity.International journal of molecular sciences · 2022Article
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- Mitogen-Activated Protein Kinase Expression Profiling Revealed Its Role in Regulating Stress Responses in Potato (Plants (Basel, Switzerland) · 2021Article
- In-silico genome wide analysis of Mitogen activated protein kinase kinase kinase gene family in C. sinensis.PloS one · 2021Article
- Transcriptome Analysis Identified Coordinated Control of Key Pathways Regulating Cellular Physiology and Metabolism uponJournal of fungi (Basel, Switzerland) · 2020Article
- Receptor-Like Protein Kinases Function Upstream of MAPKs in Regulating Plant Development.International journal of molecular sciences · 2020Review
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- Arabidopsis thaliana responds to colonisation of Piriformospora indica by secretion of symbiosis-specific proteins.PloS one · 2018Article
- Mitogen-Activated Protein Kinase Cascades in Plant Hormone Signaling.Frontiers in plant science · 2018Review
- GhMAP3K65, a Cotton Raf-Like MAP3K Gene, Enhances Susceptibility to Pathogen Infection and Heat Stress by Negatively Modulating Growth and Development in Transgenic Nicotiana benthamiana.International journal of molecular sciences · 2017Article
- The MAPKKK gene family in cassava: Genome-wide identification and expression analysis against drought stress.Scientific reports · 2017Article
- Global Identification, Classification, and Expression Analysis of MAPKKK genes: Functional Characterization of MdRaf5 Reveals Evolution and Drought-Responsive Profile in Apple.Scientific reports · 2017Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitogen-activated protein kinase (MAPK) cascades are critical signaling modules that mediate the transduction of extracellular stimuli into intracellular response. A relatively large number of MAPKKKs have been identified in a variety of plant genomes but only a few of them have been studied for their biological function. In the present study, we identified an Arabidopsis Raf-like MAPKKK gene Raf43 and studied its function in biotic and abiotic stress response using a T-DNA insertion mutant raf43-1 and two Raf43-overexpressing lines Raf43-OE#1 and Raf43-OE#13. Expression of Raf43 was induced by multiple abiotic and biotic stresses including treatments with drought, mannitol and oxidative stress or defense signaling molecule salicylic acid and infection with necrotrophic fungal pathogen Botrytis cinerea. Seed germination and seedling root growth of raf43-1 were significantly inhibited on MS medium containing mannitol, NaCl, H2O2 or methyl viologen (MV) while seed germination and seedling root growth of the Raf43-OE#1 and Raf43-OE#13 lines was similar to wild type Col-0 under the above stress conditions. Soil-grown raf43-1 plants exhibited reduced tolerance to MV, drought and salt stress. Abscisic acid inhibited significantly seed germination and seedling root growth of the raf43-1 line but had no effect on the two Raf43-overexpressing lines. Expression of stress-responsive RD17 and DREB2A genes was significantly down-regulated in raf43-1 plants. However, the raf43-1 and Raf43-overexpressing plants showed similar disease phenotype to the wild type plants after infection with B. cinerea or Pseudomonas syringae pv. tomato DC3000. Our results demonstrate that Raf43, encoding for a Raf-like MAPKKK, is required for tolerance to multiple abiotic stresses in Arabidopsis.
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