ArticlePloS one2015
A Proteomic Analysis of Individual and Gender Variations in Normal Human Urine and Cerebrospinal Fluid Using iTRAQ Quantification.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 73 citations in OpenAlex.
- Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles.Journal of extracellular vesicles · 2021Guideline
- Sex differences in urinary extracellular vesicles originating from the genitourinary system in health and disease.American journal of physiology. Renal physiology · 2026Review
- A scoping review of risk factors for urinary tract infections following renal transplantation.BMC urology · 2026Article
- Standard operating procedure combined with comprehensive quality control system for multiple LC-MS platforms urinary proteomics.Nature communications · 2025Article
- Sex discrimination from urine traces for forensic purposes using attenuated total reflectance Fourier transform infrared spectroscopy and multivariate data analysis.International journal of legal medicine · 2022Article
- Determining Plasma Protein Variation Parameters as a Prerequisite for Biomarker Studies-A TMT-Based LC-MSMS Proteome Investigation.Proteomes · 2021Article
- HBFP: a new repository for human body fluid proteome.Database : the journal of biological databases and curation · 2021Article
- Proteomics of Primary Uveal Melanoma: Insights into Metastasis and Protein Biomarkers.Cancers · 2021Article
- Proteomic analysis of human frontal and temporal cortex using iTRAQ-based 2D LC-MS/MS.Chinese neurosurgical journal · 2021Article
- Urinary proteome profiling for stratifying patients with familial Parkinson's disease.EMBO molecular medicine · 2021Article
- Association of CSF proteins with tau and amyloid β levels in asymptomatic 70-year-olds.Alzheimer's research & therapy · 2021Article
- Human body-fluid proteome: quantitative profiling and computational prediction.Briefings in bioinformatics · 2021Review
- Article
- Urinary Reference Values and First Insight into the Urinary Proteome of Captive Giraffes.Animals : an open access journal from MDPI · 2020Article
- Biomarker discovery and beyond for diagnosis of bladder diseases.Bladder (San Francisco, Calif.) · 2020Article
- Identification of Urinary Biomarkers for Exercise-Induced Immunosuppression by iTRAQ Proteomics.BioMed research international · 2020Article
- Preliminary study of the urinary proteome in Li and Han ethnic individuals from Hainan.Science China. Life sciences · 2020Article
- Early urine proteome changes in the Walker-256 tail-vein injection rat model.Scientific reports · 2019Article
- Comprehensive Analysis of Individual Variation in the Urinary Proteome Revealed Significant Gender Differences.Molecular & cellular proteomics : MCP · 2019Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Urine and cerebrospinal fluid (CSF) are two important biofluids used for disease biomarker discovery. For differential proteomic analysis, it is essential to evaluate individual and gender variations. In this study, we characterized urinary and CSF proteomes of 14 healthy volunteers with regard to individual and gender variations using 2DLC-MS/MS analysis and 8-plex iTRAQ quantification. A total of 968/512 urinary/CSF proteins were identified, with 406/280 quantified in all individuals. The median inter-individual coefficients of variation (CVs) were 0.262 and 0.183 for urinary and CSF proteomes, respectively. Cluster analysis showed that male and female urinary proteomes exhibited different patterns, though CSF proteome showed no remarkable gender differences. In comparison with CSF proteome, urinary proteome showed higher individual variation. Further analysis revealed that individual variation was not correlated with protein abundance. The minimum sample size for proteomic analysis with a 2-fold change was 10 (4/5 for males/females using iTRAQ quantification) for urinary or 8 for CSF proteome. Intracellular proteins leaked from exfoliative cells tended to have higher CVs, and extracellular proteins secreted from urinary tract or originating from plasma tended to have lower CVs. The above results might be beneficial for differential proteomic analysis and biomarker discovery.
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