Evidence map›Paper›PMID 26221250›Full record

ArticleInternational journal of clinical and experimental medicine2015

Icariin stimulates the proliferation of rat bone mesenchymal stem cells via ERK and p38 MAPK signaling.

Shuyan Qin, Wei Zhou, Shaoying Liu, Puxiang Chen, Hongjin Wu

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PubMed Full text at PMC
In one paragraph

Article in International journal of clinical and experimental medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
6.8field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 56 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 1 country.

Shuyan QinBeijing Haidian Hospital, Haidian Section of Peking University Third Hospital 29 Zhongguancun Dajie, Haidian District, Beijing 100080, China.
Wei ZhouDepartment of Orthopedic Microsurgery, Taihe Hospital, Hubei University of Medicine 32 Renmin Nan Road, Shiyan 442000, China.
Shaoying LiuBeijing Hospital of Integrated Traditional Chinese and Western Medicine Beijing, China.
Puxiang ChenDepartment of Gynecology and Obstetrics, The Second Xiangya Hospital, Central South University 139 Renmin Zhong Road, Changsha 410011, China.
Hongjin WuBeijing Haidian Hospital, Haidian Section of Peking University Third Hospital 29 Zhongguancun Dajie, Haidian District, Beijing 100080, China.
Beijing Haidian Hospital · CNBeijing Chang'an Integrated Traditional Chinese and Western Medicine Hospital · CNCentral South University · CNHubei University of Medicine · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bone mesenchymal stem cells (BMSCs) are able to differentiate into multi types of lineages, so they have been widely applied in the stem cell transplantation. The BMSCs are usually needed to be expanded before transplantation due to their limited content in bone marrow. It has recently been reported that Icariin (ICA), a major constituent of flavonoids from the Chinese medical herb Epimedium brevicornum Maxim, promotes the proliferation of various types of differentiated cells. However, whether ICA can enhance BMSCs proliferation and the possible underlying mechanisms are still unknown. After being isolated and purified from rat bone marrow, cultured BMSCs are stimulated with different concentrations of ICA. The cytotoxicity of ICA is evaluated by the Cell Counting Kit-8 (CCK-8) assay method and the ICA optimal concentration for BMSCs proliferation is determined at 320 μg/L. Our work reveals that ICA induces an obvious phosphorylation of ERK and p38 kinases in BMSCs, no matter serum exists or not. Inhibition of ERK or p38 MAPK signaling by their specific inhibitors PD98059 or SP600125, respectively, not only prevents the activation of these kinases, but also attenuates cell proliferation induced by ICA. Furthermore, the downstream transcription factors of MAPK pathway, Elk1, Stat3, c-Myc and Fos, are also monitored by RT-PCR, and our results show that among them, Elk1 and c-Myc are significantly upregulated after ICA treatment. Taken together, our results demonstrate that ICA promotes the proliferation of rat BMSCs through activating ERK and p38 MAPK signaling which further leads to upregulation of their downstream transcription factors Elk1 and c-Myc. Our work provides a novel effective way to expand the content of BMSCs in vitro, which casts light on clinical applications of stem cell transplantation in the future.

Indexed as

bone mesenchymal stem cellsERKIcariinp38 MAPK

Identifiers

PMID26221250
PMCPMC4509195
OpenAlexW1029875035

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.