Evidence map›Paper›PMID 26221095›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2015

A high-throughput screening assay for distinguishing nitrile hydratases from nitrilases.

Leticia Mara Lima Angelini, Amanda Ribeiro Martins da Silva, Lucas de Freitas Coli Rocco, Cintia Duarte de Freitas Milagre

Abstract readEvaluation Study
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Leticia Mara Lima AngeliniInstituto de of Biociências, Universidade Estadual Paulista "Julio de Mesquita Filho", Rio Claro, SP, Brazil.
Amanda Ribeiro Martins da SilvaInstituto de of Biociências, Universidade Estadual Paulista "Julio de Mesquita Filho", Rio Claro, SP, Brazil.
Lucas de Freitas Coli RoccoInstituto de of Biociências, Universidade Estadual Paulista "Julio de Mesquita Filho", Rio Claro, SP, Brazil.
Cintia Duarte de Freitas MilagreDepartamento de Química Orgânia, Instituto de Química, Universidade Estadual Paulista "Julio de Mesquita Filho", Araraquara, SP, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A modified colorimetric high-throughput screen based on pH changes combined with an amidase inhibitor capable of distinguishing between nitrilases and nitrile hydratases. This enzymatic screening is based on a binary response and is suitable for the first step of hierarchical screening projects.

Indexed as

AmidohydrolasesAminohydrolasesColorimetryHigh-Throughput Screening AssaysHydrogen-Ion ConcentrationHydro-LyasesamidaseAmidohydrolasesAminohydrolasesHydro-Lyasesnitrilasenitrile hydrataseamidaseamidase inhibitorhigh-throughput screeningnitrilasenitrile hydratase

Identifiers

PMID26221095
PMCPMC4512080

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.