ArticleInternational journal of biological sciences2015
Cathepsin B Cysteine Proteinase is Essential for the Development and Pathogenesis of the Plant Parasitic Nematode Radopholus similis.
Article in International journal of biological sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
- The pinewood nematode enhances population growth by exploiting host defensive terpenes via lysosomal signaling.Pest management science · 2026Article
- Biochemical and nanotechnological approaches to combat phytoparasitic nematodes.Plant biotechnology journal · 2024Review
- Silencing Ditylenchus destructor cathepsin L-like cysteine protease has negative pleiotropic effect on nematode ontogenesis.Scientific reports · 2024Article
- Transgenic East African Highland Banana Plants Are Protected againstInternational journal of molecular sciences · 2023Article
- Single Nematode Transcriptomic Analysis, Using Long-Read Technology, Reveals Two Novel Virulence Gene Candidates in the Soybean Cyst Nematode,International journal of molecular sciences · 2023Article
- Protein and antigen profiles of third-stage larvae of Gnathostoma spinigerum assessed with next-generation sequencing transcriptomic information.Scientific reports · 2022Article
- RNA Interference for Improving Disease Resistance in Plants and Its Relevance in This Clustered Regularly Interspaced Short Palindromic Repeats-Dominated Era in Terms of dsRNA-Based Biopesticides.Frontiers in plant science · 2022Review
- Targeted transcriptomics reveals signatures of large-scale independent origins and concerted regulation of effector genes in Radopholus similis.PLoS pathogens · 2021Article
- A novel ATPase gene, Ab-atps, plays an important role in the interaction of rice and white tip nematode, Aphelenchoides besseyi.Scientific reports · 2021Article
- A Venom Allergen-Like Protein, RsVAP, the First Discovered Effector Protein ofInternational journal of molecular sciences · 2021Article
- Current Insights into Migratory Endoparasitism: Deciphering the Biology, Parasitism Mechanisms, and Management Strategies of Key Migratory Endoparasitic Phytonematodes.Plants (Basel, Switzerland) · 2020Review
- Investigation of three enzymes and their roles in the embryonic development of parthenogenetic Haemaphysalis longicornis.Parasites & vectors · 2020Article
- Multi-copy alpha-amylase genes are crucial for Ditylenchus destructor to parasitize the plant host.PloS one · 2020Article
- Life-stage specific transcriptomes of a migratory endoparasitic plant nematode, Radopholus similis elucidate a different parasitic and life strategy of plant parasitic nematodes.Scientific reports · 2019Article
- RNAi-mediated protection against banana diseases and pests.3 Biotech · 2019Review
- The genome of the migratory nematode, Radopholus similis, reveals signatures of close association to the sedentary cyst nematodes.PloS one · 2019Article
- Article
- Rational design of biosafe crop resistance to a range of nematodes using RNA interference.Plant biotechnology journal · 2018Article
- Article
- Transgenic Nicotiana benthamiana plants expressing a hairpin RNAi construct of a nematode Rs-cps gene exhibit enhanced resistance to Radopholus similis.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Radopholus similis is an important plant parasitic nematode which severely harms many crops. Cathepsin B is present in a wide variety of organisms, and plays an important role in many parasites. Understanding cathepsin B of R. similis would allow us to find new targets and approaches for its control. In this study, we found that Rs-cb-1 mRNA was expressed in esophageal glands, intestines and gonads of females, testes of males, juveniles and eggs in R. similis. Rs-cb-1 expression was the highest in females, followed by juveniles and eggs, and was the lowest in males. The maximal enzyme activity of Rs-CB-1 was detected at pH 6.0 and 40 °C. Silencing of Rs-cb-1 using in vitro RNAi (Soaking with dsRNA in vitro) not only significantly inhibited the development and hatching of R. similis, but also greatly reduced its pathogenicity. Using in planta RNAi, we confirmed that Rs-cb-1 expression in nematodes were significantly suppressed and the resistance to R. similis was significantly improved in T2 generation transgenic tobacco plants expressing Rs-cb-1 dsRNA. The genetic effects of in planta RNAi-induced gene silencing could be maintained in the absence of dsRNA for at least two generations before being lost, which was not the case for the effects induced by in vitro RNAi. Overall, our results first indicate that Rs-cb-1 plays key roles in the development, hatching and pathogenesis of R. similis, and that in planta RNAi is an effective tool in studying gene function and genetic engineering of plant resistance to migratory plant parasitic nematodes.
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