Evidence map›Paper›PMID 26220954›Full record

ArticlePlant physiology2015

The Use of Contact Mode Atomic Force Microscopy in Aqueous Medium for Structural Analysis of Spinach Photosynthetic Complexes.

Witchukorn Phuthong, Zubin Huang, Tyler M Wittkopp, Kinga Sznee, Mark L Heinnickel, Jan P Dekker, Raoul N Frese, Fritz B Prinz, Arthur R Grossman

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 31 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Witchukorn PhuthongDepartment of Materials Science and Engineering (W.P., F.B.P.), Department of Mechanical Engineering (Z.H., F.B.P.), and Department of Biology (T.M.W.), Stanford University, Stanford, California 94305;Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305 (T.M.W., M.L.H., A.R.G.); andDepartment of Physics and Astronomy, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands (K.S., J.P.D., R.N.F.) phuthong@stanford.edu.ORCID http://orcid.org/0000-0002-9811-3251
Zubin HuangDepartment of Materials Science and Engineering (W.P., F.B.P.), Department of Mechanical Engineering (Z.H., F.B.P.), and Department of Biology (T.M.W.), Stanford University, Stanford, California 94305;Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305 (T.M.W., M.L.H., A.R.G.); andDepartment of Physics and Astronomy, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands (K.S., J.P.D., R.N.F.).
Tyler M WittkoppDepartment of Materials Science and Engineering (W.P., F.B.P.), Department of Mechanical Engineering (Z.H., F.B.P.), and Department of Biology (T.M.W.), Stanford University, Stanford, California 94305;Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305 (T.M.W., M.L.H., A.R.G.); andDepartment of Physics and Astronomy, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands (K.S., J.P.D., R.N.F.).
Kinga SzneeDepartment of Materials Science and Engineering (W.P., F.B.P.), Department of Mechanical Engineering (Z.H., F.B.P.), and Department of Biology (T.M.W.), Stanford University, Stanford, California 94305;Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305 (T.M.W., M.L.H., A.R.G.); andDepartment of Physics and Astronomy, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands (K.S., J.P.D., R.N.F.).
Mark L HeinnickelDepartment of Materials Science and Engineering (W.P., F.B.P.), Department of Mechanical Engineering (Z.H., F.B.P.), and Department of Biology (T.M.W.), Stanford University, Stanford, California 94305;Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305 (T.M.W., M.L.H., A.R.G.); andDepartment of Physics and Astronomy, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands (K.S., J.P.D., R.N.F.).
Jan P DekkerDepartment of Materials Science and Engineering (W.P., F.B.P.), Department of Mechanical Engineering (Z.H., F.B.P.), and Department of Biology (T.M.W.), Stanford University, Stanford, California 94305;Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305 (T.M.W., M.L.H., A.R.G.); andDepartment of Physics and Astronomy, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands (K.S., J.P.D., R.N.F.).
Raoul N FreseDepartment of Materials Science and Engineering (W.P., F.B.P.), Department of Mechanical Engineering (Z.H., F.B.P.), and Department of Biology (T.M.W.), Stanford University, Stanford, California 94305;Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305 (T.M.W., M.L.H., A.R.G.); andDepartment of Physics and Astronomy, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands (K.S., J.P.D., R.N.F.).
Fritz B PrinzDepartment of Materials Science and Engineering (W.P., F.B.P.), Department of Mechanical Engineering (Z.H., F.B.P.), and Department of Biology (T.M.W.), Stanford University, Stanford, California 94305;Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305 (T.M.W., M.L.H., A.R.G.); andDepartment of Physics and Astronomy, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands (K.S., J.P.D., R.N.F.).
Arthur R GrossmanDepartment of Materials Science and Engineering (W.P., F.B.P.), Department of Mechanical Engineering (Z.H., F.B.P.), and Department of Biology (T.M.W.), Stanford University, Stanford, California 94305;Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305 (T.M.W., M.L.H., A.R.G.); andDepartment of Physics and Astronomy, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands (K.S., J.P.D., R.N.F.).
Carnegie Institution for Science · USStanford University · USVrije Universiteit Amsterdam · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To investigate the dynamics of photosynthetic pigment-protein complexes in vascular plants at high resolution in an aqueous environment, membrane-protruding oxygen-evolving complexes (OECs) associated with photosystem II (PSII) on spinach (Spinacia oleracea) grana membranes were examined using contact mode atomic force microscopy. This study represents, to our knowledge, the first use of atomic force microscopy to distinguish the putative large extrinsic loop of Photosystem II CP47 reaction center protein (CP47) from the putative oxygen-evolving enhancer proteins 1, 2, and 3 (PsbO, PsbP, and PsbQ) and large extrinsic loop of Photosystem II CP43 reaction center protein (CP43) in the PSII-OEC extrinsic domains of grana membranes under conditions resulting in the disordered arrangement of PSII-OEC particles. Moreover, we observed uncharacterized membrane particles that, based on their physical characteristics and electrophoretic analysis of the polypeptides associated with the grana samples, are hypothesized to be a domain of photosystem I that protrudes from the stromal face of single thylakoid bilayers. Our results are interpreted in the context of the results of others that were obtained using cryo-electron microscopy (and single particle analysis), negative staining and freeze-fracture electron microscopy, as well as previous atomic force microscopy studies.

Indexed as

Image EnhancementMicroscopy, Atomic ForceMultiprotein ComplexesPhotosystem II Protein ComplexSpinacia oleraceaThylakoidsMultiprotein ComplexesPhotosystem II Protein Complex

Identifiers

PMID26220954
PMCPMC4587457
OpenAlexW2103975222

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.