Evidence map›Paper›PMID 26219403›Full record

ArticleAnnals of biomedical engineering2016

Effects of Osmolarity on the Spontaneous Calcium Signaling of In Situ Juvenile and Adult Articular Chondrocytes.

Yilu Zhou, Michael A David, Xingyu Chen, Leo Q Wan, Randall L Duncan, Liyun Wang, X Lucas Lu

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of biomedical engineering, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.3field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 30 citations in OpenAlex.

  1. Article
  2. Primary cilium-mediated mechanotransduction in cartilage chondrocytes.Experimental biology and medicine (Maywood, N.J.) · 2023
    Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Roles of TRPV4 and piezo channels in stretch-evoked CaExperimental biology and medicine (Maywood, N.J.) · 2020
    Article
  8. Spontaneous calcium signaling of cartilage cells: from spatiotemporal features to biophysical modeling.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019
    Article
  9. Article
  10. Article
  11. Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Yilu ZhouDepartment of Mechanical Engineering, University of Delaware, 130 Academy Street SPL126, Newark, DE, 19716, USA.
Michael A DavidDepartment of Biomedical Engineering, University of Delaware, Newark, DE, 19716, USA.
Xingyu ChenDepartment of Mechanical Engineering, University of Delaware, 130 Academy Street SPL126, Newark, DE, 19716, USA.
Leo Q WanDepartment of Biomedical Engineering, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA.
Randall L DuncanDepartment of Biological Sciences, University of Delaware, Newark, DE, 19716, USA.
Liyun WangDepartment of Mechanical Engineering, University of Delaware, 130 Academy Street SPL126, Newark, DE, 19716, USA.
X Lucas LuDepartment of Mechanical Engineering, University of Delaware, 130 Academy Street SPL126, Newark, DE, 19716, USA. xlu@udel.edu.
University of Delaware · USRensselaer Polytechnic Institute · US

Funding

Subproject Title: Clinical Research Education, Mentoring and Career Development CoreU54GM104941 · NIGMS · UNIVERSITY OF DELAWARE · PI Gregory E Hicks · 2013 to 2026
$60.5M
NIGMS NIH HHS U54 GM104941NIGMS NIH HHS U54-GM104941
6 · The paper itself

Abstract

Calcium is a universal second messenger that mediates the metabolic activity of chondrocytes in articular cartilage. Spontaneous intracellular calcium ([Ca(2+)]i) oscillations, similar to those in neurons and myocytes, have recently been observed in chondrocytes. This study analyzed and compared the effects of different osmotic environments (hypertonic, hypotonic, and isotonic) on the spontaneous [Ca(2+)]i signaling of in situ chondrocytes residing in juvenile and adult cartilage explants. In spite of a lower cell density, a significantly higher percentage of chondrocytes in adult cartilage under all osmotic environments demonstrated spontaneous [Ca(2+)]i oscillations than chondrocytes in juvenile cartilage. For both juvenile and adult chondrocytes, hypotonic stress increased while hypertonic stress decreased the response rates. Furthermore, the spatiotemporal characteristics of the [Ca(2+)]i peaks vary in an age-dependent manner. In the hypotonic environment, the [Ca(2+)]i oscillation frequency of responsive adult cells is almost tripled whereas the juvenile cells respond with an increased duration and magnitude of each [Ca(2+)]i peak. Both juvenile and adult chondrocytes demonstrated significantly slower [Ca(2+)]i oscillations with longer rising and recovery time under the hypertonic condition. Taken together, these results shed new insights into the interplay between age and osmotic environment that may regulate the fundamental metabolism of chondrocytes.

Indexed as

Calcium SignalingOsmolar ConcentrationAge FactorsAnimalsCartilage, ArticularCattleChondrocytesAgeCalciumCartilageChondrocyteOsmoticSignal transduction

Identifiers

PMID26219403
PMCPMC4732936
OpenAlexW960888796

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.