ArticlePloS one2015
Clinical Specimen-Direct LAMP: A Useful Tool for the Surveillance of blaOXA-23-Positive Carbapenem-Resistant Acinetobacter baumannii.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.
- Emerging strategies for the noninvasive diagnosis of nosocomial pneumonia.Expert review of anti-infective therapy · 2019Pooled it
- Development of Loop-Mediated Isothermal Amplification with a Lateral Flow Dipstick for Detection of theInternational journal of molecular sciences · 2026Article
- Current Methods for Reliable Identification of Species in theMicroorganisms · 2025Review
- Development of an RT-LAMP Assay for DetectingDiagnostics (Basel, Switzerland) · 2025Article
- Laboratory detection of carbapenemases among Gram-negative organisms.Clinical microbiology reviews · 2024Review
- A novel machine-learning aided platform for rapid detection of urine ESBLs and carbapenemases: URECA-LAMP.Journal of clinical microbiology · 2024Article
- Current and Future Technologies for the Detection of Antibiotic-Resistant Bacteria.Diagnostics (Basel, Switzerland) · 2023Review
- The Prevalence of Multidrug-ResistantVaccines · 2023Review
- Development of a Real-Time Recombinase-Aided Amplification Method to Rapidly Detect Methicillin-ResistantMicroorganisms · 2022Article
- Real-time optical analysis of a colorimetric LAMP assay for SARS-CoV-2 in saliva with a handheld instrument improves accuracy compared with endpoint assessment.Journal of biomolecular techniques : JBT · 2021Article
- Development of Loop-Mediated Isothermal Amplification Assay for Detection of Clinically Significant Members ofFrontiers in molecular biosciences · 2021Article
- Current state of the art in rapid diagnostics for antimicrobial resistance.Lab on a chip · 2020Review
- Identification of Acinetobacter baumannii and its carbapenem-resistant gene blaScientific reports · 2019Article
- Advances in Directly Amplifying Nucleic Acids from Complex Samples.Biosensors · 2019Review
- Rapid screening and early precautions for carbapenem-resistantAntimicrobial resistance and infection control · 2019Article
- New Molecular Diagnostic Approaches to Bacterial Infections and Antibacterial Resistance.Annual review of medicine · 2018Review
- Article
- Surveillance of Dihydropteroate Synthase Genes inFrontiers in microbiology · 2016Article
- Why is Acinetobacter baumannii a problem for critically ill patients?Intensive care medicine · 2015Article
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Healthcare-associated infections are a leading cause of morbidity and mortality worldwide. Treatment is increasingly complicated by the escalating incidence of antimicrobial resistance. Among drug-resistant pathogens, carbapenem-resistant Acinetobacter baumannii (CRAb) is of increasing concern because of the limited applicable therapies and its expanding global distribution in developed countries and newly industrialized countries. Therefore, a rapid detection method that can be used even in resource-poor countries is urgently required to control this global public health threat. Conventional techniques, such as bacterial culture and polymerase chain reaction (PCR), are insufficient to combat this threat because they are time-consuming and laborious. In this study, we developed a loop-mediated isothermal amplification (LAMP) method for detecting blaOXA-23-positive CRAb, the most prevalent form of CRAb in Asia, especially in Thailand, and confirmed its efficacy as a surveillance tool in a clinical setting. Clinical samples of sputum and rectal swabs were collected from patients in a hospital in Bangkok and used for LAMP assays. After boiling and centrifugation, the supernatants were used directly in the assay. In parallel, a culture method was used for comparison purposes to evaluate the specificity and sensitivity of LAMP. As a first step, a total of 120 sputum samples were collected. The sensitivity of LAMP was 88.6% (39/44), and its specificity was 92.1% (70/76) using the culture method as the "gold standard". When surveillance samples including sputum and rectal swabs were analyzed with the LAMP assay, its sensitivity was 100.0%. This method enables the direct analysis of clinical specimens and provides results within 40 minutes of sample collection, making it a useful tool for surveillance even in resource-poor countries.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.