Evidence map›Paper›PMID 26218530›Full record

Trial reportPloS one2015

Characterization of the Microenvironment in Positive and Negative Sentinel Lymph Nodes from Melanoma Patients.

Meriem Messaoudene, Aurélie Périer, Giulia Fregni, Emmanuelle Neves, Laurence Zitvogel, Isabelle Cremer, Johan Chanal, Xavier Sastre-Garau, Lydia Deschamps, Eduardo Marinho and 4 more

Registry-linked trialOpen access · goldAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT01336270 (Characterisation of T Lymphocytes, NK Cells and Macrophages in Melanoma Patients), which is not on this map. Cited by 11 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed, 1 pooled it
1.0field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT01336270 completednot on this map

Characterisation of T Lymphocytes, NK Cells and Macrophages in Melanoma Patients: Impact on Immunity Versus Immunosuppression

TypeobservationalSponsorAssistance Publique - Hôpitaux de ParisRan2011 to 2014Enrolled217ConditionsMelanomaArmsbiological collection
3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 1 synthesis or guideline pooled it, 16 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
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  5. Review
  6. Memory-like Differentiation Enhances NK Cell Responses to Melanoma.Clinical cancer research : an official journal of the American Association for Cancer Research · 2021
    Article
  7. Review
  8. Article
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  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 7 institutions in 1 country.

Meriem MessaoudeneINSERMU1160, Institut Universitaire d'Hématologie, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75010, Paris, France.
Aurélie PérierU1015 INSERM-CIC, Institut Gustave Roussy, Villejuif, France.
Giulia FregniINSERMU1160, Institut Universitaire d'Hématologie, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75010, Paris, France.
Emmanuelle NevesINSERMU1160, Institut Universitaire d'Hématologie, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75010, Paris, France.
Laurence ZitvogelU1015 INSERM-CIC, Institut Gustave Roussy, Villejuif, France.
Isabelle CremerCentre de Recherche des Cordeliers, 15, rue de l'école de Médecine, 75006, Paris, France.
Johan ChanalAPHP, Department of Dermatology, Hospital Cochin, University Paris Descartes, Paris, France.
Xavier Sastre-GarauBiopathology Department, Institut Curie, Rue d'Ulm, Paris, France.
Lydia DeschampsAPHP, Department of Dermatology and Department of Pathology, Hospital Bichat, University Paris Diderot, Hospital Bichat, 75018, Paris, France.
Eduardo MarinhoAPHP, Department of Dermatology and Department of Pathology, Hospital Bichat, University Paris Diderot, Hospital Bichat, 75018, Paris, France.
Frederique LarousserieAPHP, Department of Pathology, Hospital Cochin, University Paris Descartes, Paris, France.
Eve MaubecAPHP, Department of Dermatology and Department of Pathology, Hospital Bichat, University Paris Diderot, Hospital Bichat, 75018, Paris, France.
Marie-Françoise AvrilAPHP, Department of Dermatology, Hospital Cochin, University Paris Descartes, Paris, France.
Anne CaignardINSERMU1160, Institut Universitaire d'Hématologie, Hôpital Saint Louis, 1 Avenue Claude Vellefaux, 75010, Paris, France.
Assistance Publique – Hôpitaux de Paris · FRInserm · FRDélégation Paris 5 · FRHôpital Saint-Louis · FRCentre de Recherche des Cordeliers · FRInstitut Curie · FRInstitut Gustave Roussy · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Melanomas are aggressive skin tumors characterized by high metastatic potential. Our previous results indicate that Natural Killer (NK) cells may control growth of melanoma. The main defect of blood NK cells was a decreased expression of activating NCR1/NKp46 receptor and a positive correlation of NKp46 expression with disease outcome in stage IV melanoma patients was found. In addition, in stage III melanoma patients, we identified a new subset of mature NK cells in macro-metastatic Lymph nodes (LN). In the present studies, we evaluated the numbers of NK cells infiltrating primary cutaneous melanoma and analyzed immune cell subsets in a series of sentinel lymph nodes (SLN). First, we show that NKp46+ NK cells infiltrate primary cutaneous melanoma. Their numbers were related to age of patients and not to Breslow thickness. Then, a series of patients with tumor-negative or -positive sentinel lymph nodes matched for Breslow thickness of the cutaneous melanoma was constituted. We investigated the distribution of macrophages (CD68), endothelial cells, NK cells, granzyme B positive (GrzB+) cells and CD8+ T cells in the SLN. Negative SLN (SLN-) were characterized by frequent adipose involution and follicular hyperplasia compared to positive SLN (SLN+). High densities of macrophages and endothelial cells (CD34), prominent in SLN+, infiltrate SLN and may reflect a tumor favorable microenvironment. Few but similar numbers of NK and GrzB+ cells were found in SLN- and SLN+: NK cells and GrzB+ cells were not correlated. Numerous CD8+ T cells infiltrated SLN with a trend for higher numbers in SLN-. Moreover, CD8+ T cells and GrzB+ cells correlated in SLN- not in SLN+. We also observed that the numbers of CD8+ T cells negatively correlated with endothelial cells in SLN-. The numbers of NK, GrzB+ or CD8+ T cells had no significant impact on overall survival. However, we found that the 5 year-relapse rate was higher in SLN with higher numbers of NK cells.

Indexed as

Sentinel Lymph Node BiopsyTumor MicroenvironmentAdultAgedAged, 80 and overAntigens, DifferentiationCD8-Positive T-LymphocytesEndothelial CellsFemaleHumansKiller Cells, NaturalMacrophagesMaleMelanomaMiddle AgedNeoplasm StagingAntigens, Differentiation

Identifiers

PMID26218530
PMCPMC4517810
OpenAlexW2201036353

What OpenQuestion holds

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.