ArticlePloS one2015
Inactivation of ca10a and ca10b Genes Leads to Abnormal Embryonic Development and Alters Movement Pattern in Zebrafish.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 20 citations in OpenAlex.
- Unveiling Carbonic Anhydrase VIII, X, XI Expression in Cancer and Neurological Diseases Through Integrated Bioinformatics Approaches.Bioinformatics and biology insights · 2026Article
- Applications of Carbonic Anhydrase Inhibitors in Neurological Disorders, Mechanisms and Therapeutic Potential.Sub-cellular biochemistry · 2026Review
- Making sense of carbonic anhydrase function in zebrafish using antisense morpholinos.Molecular genetics and genomics : MGG · 2025Review
- Novel Drug Screening Assay forJournal of medicinal chemistry · 2024Article
- Roles of Carbonic Anhydrases and Carbonic Anhydrase Related Proteins in Zebrafish.International journal of molecular sciences · 2022Review
- Application of beta and gamma carbonic anhydrase sequences as tools for identification of bacterial contamination in the whole genome sequence of inbred Wuzhishan minipig (Sus scrofa) annotated in databases.Database : the journal of biological databases and curation · 2021Article
- CA10 regulates neurexin heparan sulfate addition via a direct binding in the secretory pathway.EMBO reports · 2021Article
- Assessment of databases to determine the validity of β- and γ-carbonic anhydrase sequences from vertebrates.BMC genomics · 2020Article
- Catalytically inactive carbonic anhydrase-related proteins enhance transport of lactate by MCT1.FEBS open bio · 2019Article
- Intelectin 3 is dispensable for resistance against a mycobacterial infection in zebrafish (Danio rerio).Scientific reports · 2019Article
- Carbonic anhydrase related protein expression in astrocytomas and oligodendroglial tumors.BMC cancer · 2018Article
- Chromatin accessibility is associated with CRISPR-Cas9 efficiency in the zebrafish (Danio rerio).PloS one · 2018Article
- Carbonic anhydrase-related protein CA10 is an evolutionarily conserved pan-neurexin ligand.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Expression of Carbonic Anhydrase I in Motor Neurons and Alterations in ALS.International journal of molecular sciences · 2016Article
- Zebrafish Models of Prader-Willi Syndrome: Fast Track to Pharmacotherapeutics.Diseases (Basel, Switzerland) · 2016Article
- Function Over Form: Modeling Groups of Inherited Neurological Conditions in Zebrafish.Frontiers in molecular neuroscience · 2016Review
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carbonic anhydrase related proteins (CARPs) X and XI are highly conserved across species and are predominantly expressed in neural tissues. The biological role of these proteins is still an enigma. Ray-finned fish have lost the CA11 gene, but instead possess two co-orthologs of CA10. We analyzed the expression pattern of zebrafish ca10a and ca10b genes during embryonic development and in different adult tissues, and studied 61 CARP X/XI-like sequences to evaluate their phylogenetic relationship. Sequence analysis of zebrafish ca10a and ca10b reveals strongly predicted signal peptides, N-glycosylation sites, and a potential disulfide, all of which are conserved, suggesting that all of CARP X and XI are secretory proteins and potentially dimeric. RT-qPCR showed that zebrafish ca10a and ca10b genes are expressed in the brain and several other tissues throughout the development of zebrafish. Antisense morpholino mediated knockdown of ca10a and ca10b showed developmental delay with a high rate of mortality in larvae. Zebrafish morphants showed curved body, pericardial edema, and abnormalities in the head and eye, and there was increased apoptotic cell death in the brain region. Swim pattern showed abnormal movement in morphant zebrafish larvae compared to the wild type larvae. The developmental phenotypes of the ca10a and ca10b morphants were confirmed by inactivating these genes with the CRISPR/Cas9 system. In conclusion, we introduce a novel zebrafish model to investigate the mechanisms of CARP Xa and CARP Xb functions. Our data indicate that CARP Xa and CARP Xb have important roles in zebrafish development and suppression of ca10a and ca10b expression in zebrafish larvae leads to a movement disorder.
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