ArticleScientific reports2015
Melting Temperature Mapping Method: A Novel Method for Rapid Identification of Unknown Pathogenic Microorganisms within Three Hours of Sample Collection.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 27 citations in OpenAlex.
- Treatment strategies for intra-amniotic infection and/or inflammation in preterm labor cases.European journal of obstetrics & gynecology and reproductive biology: X · 2025Article
- Spontaneous Bacterial Peritonitis in Advanced Cirrhosis: Diagnosis by Tm Mapping and Inflammatory Profiles of Extracellular Vesicles.Journal of clinical medicine · 2025Article
- Melting temperature mapping method using imperfect-match linear long probes.Scientific reports · 2024Article
- Novel rapid method for identifying and quantifying pathogenic bacteria within four hours of blood collection.Scientific reports · 2024Article
- Predictive value of cell population data with Sysmex XN-series hematology analyzer for culture-proven bacteremia.Frontiers in medicine · 2023Article
- Prospective Study of the Detection of Bacterial Pathogens in Pediatric Clinical Specimens Using the Melting Temperature Mapping Method.Microbiology spectrum · 2022Article
- Infected simple renal cyst due to Streptococcus pneumoniae rapidly diagnosed by the melting temperature mapping method: a case report.BMC pediatrics · 2021Article
- A Rapid ATP Bioluminescence-based Test for Detecting Levofloxacin Resistance Starting from Positive Blood Culture Bottles.Scientific reports · 2019Article
- Spondylodiscitis due to Parvimonas micra diagnosed by the melting temperature mapping method: a case report.BMC infectious diseases · 2017Article
- Microbial Typing by Machine Learned DNA Melt Signatures.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acquiring the earliest possible identification of pathogenic microorganisms is critical for selecting the appropriate antimicrobial therapy in infected patients. We herein report the novel "melting temperature (Tm) mapping method" for rapidly identifying the dominant bacteria in a clinical sample from sterile sites. Employing only seven primer sets, more than 100 bacterial species can be identified. In particular, using the Difference Value, it is possible to identify samples suitable for Tm mapping identification. Moreover, this method can be used to rapidly diagnose the absence of bacteria in clinical samples. We tested the Tm mapping method using 200 whole blood samples obtained from patients with suspected sepsis, 85% (171/200) of which matched the culture results based on the detection level. A total of 130 samples were negative according to the Tm mapping method, 98% (128/130) of which were also negative based on the culture method. Meanwhile, 70 samples were positive according to the Tm mapping method, and of the 59 suitable for identification, 100% (59/59) exhibited a "match" or "broad match" with the culture or sequencing results. These findings were obtained within three hours of whole blood collection. The Tm mapping method is therefore useful for identifying infectious diseases requiring prompt treatment.
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