ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2015
Novel full-spectral flow cytometry with multiple spectrally-adjacent fluorescent proteins and fluorochromes and visualization of in vivo cellular movement.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 127 citations in OpenAlex.
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- CaptureBody - an anti-CD45 × anti-IgG bispecific antibody enables accurate unmixing for spectral flow cytometry.bioRxiv : the preprint server for biology · 2026Article
- Nuclear Histone 3 Post-Translational Modification Profiling in Whole Cells using Spectral Flow Cytometry.bioRxiv : the preprint server for biology · 2026Article
- Capturing the full spectrum of T cell responses with spectral flow cytometry.Oxford open immunology · 2026Review
- Article
- Beyond the Limits: How Is Spectral Flow Cytometry Reshaping the Clinical Landscape and What Is Coming Next?Cells · 2025Review
- Aryl Hydrocarbon Receptor Activation Promotes Effector CD4+ T Cell Homeostasis and Restrains Salt-Sensitive Hypertension.Function (Oxford, England) · 2025Article
- Characterization of Bone Marrow-Derived Immune Compartment in Murine Brain Metastases by Spectral Flow Cytometry.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Postbiotics: a perspective on their quantification.Frontiers in nutrition · 2025Article
- Characterisation and hierarchy of the spermatogonial stem cell compartment in human spermatogenesis by spectral cytometry using a 16-colors panel.Cellular and molecular life sciences : CMLS · 2024Article
- Development of a Spectral Flow Cytometry Analysis Pipeline for High-dimensional Immune Cell Characterization.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Using Spectral Flow Cytometry for CAR T-Cell Clinical Trials: Game Changing Technologies Enabling Novel Therapies.International journal of molecular sciences · 2024Review
- Spectral Algal Fingerprinting and Long Sequencing in Synthetic Algal-Microbial Communities.Cells · 2024Article
- Development of a Spectral Flow Cytometry Analysis Pipeline for High-Dimensional Immune Cell Characterization.bioRxiv : the preprint server for biology · 2024Article
- Understanding Spondyloarthritis Pathogenesis: The Promise of Single-Cell Profiling.Current rheumatology reports · 2024Review
- Multiparameter Flow Cytometry Monitoring of T Cell Responses.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Beyond 40 fluorescent probes for deep phenotyping of blood mononuclear cells, using spectral technology.Frontiers in immunology · 2024Article
- Prism Design for Spectral Flow Cytometry.Micromachines · 2023Article
- Advanced immunophenotyping: A powerful tool for immune profiling, drug screening, and a personalized treatment approach.Frontiers in immunology · 2023Article
- Development of Spectral Imaging Cytometry.Methods in molecular biology (Clifton, N.J.) · 2023Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flow cytometric analysis with multicolor fluoroprobes is an essential method for detecting biological signatures of cells. Here, we present a new full-spectral flow cytometer (spectral-FCM). Unlike conventional flow cytometer, this spectral-FCM acquires the emitted fluorescence for all probes across the full-spectrum from each cell with 32 channels sequential PMT unit after dispersion with prism, and extracts the signals of each fluoroprobe based on the spectral shape of each fluoroprobe using unique algorithm in high speed, high sensitive, accurate, automatic and real-time. The spectral-FCM detects the continuous changes in emission spectra from green to red of the photoconvertible protein, KikGR with high-spectral resolution and separates spectrally-adjacent fluoroprobes, such as FITC (Emission peak (Em) 519 nm) and EGFP (Em 507 nm). Moreover, the spectral-FCM can measure and subtract autofluorescence of each cell providing increased signal-to-noise ratios and improved resolution of dim samples, which leads to a transformative technology for investigation of single cell state and function. These advances make it possible to perform 11-color fluorescence analysis to visualize movement of multilinage immune cells by using KikGR-expressing mice. Thus, the novel spectral flow cytometry improves the combinational use of spectrally-adjacent various FPs and multicolor fluorochromes in metabolically active cell for the investigation of not only the immune system but also other research and clinical fields of use.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.