ArticleFrontiers in cellular and infection microbiology2015
Identification and characterization of three Vibrio alginolyticus non-coding RNAs involved in adhesion, chemotaxis, and motility processes.
Article in Frontiers in cellular and infection microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Review
- Contact-mediated algicidal mechanism ofiScience · 2024Article
- Comprehensive insights into the metabolism characteristics of small RNA Qrr4 in Vibrio alginolyticus.Applied microbiology and biotechnology · 2023Article
- Virulence regulation of ZnFrontiers in veterinary science · 2023Article
- Potential dsRNAs can be delivered to aquatic for defense pathogens.Frontiers in bioengineering and biotechnology · 2022Review
- The regulation of oxidative phosphorylation pathway on Vibrio alginolyticus adhesion under adversities.MicrobiologyOpen · 2019Article
- Mechanisms underlying the virulence regulation of newEmerging microbes & infections · 2019Article
- Regulatory role of the RstB-RstA system in adhesion, biofilm production, motility, and hemolysis.MicrobiologyOpen · 2018Article
- The complete genome sequence of Vibrio aestuarianus W-40 reveals virulence factor genes.MicrobiologyOpen · 2018Article
- secA, secD, secF, yajC, and yidC contribute to the adhesion regulation of Vibrio alginolyticus.MicrobiologyOpen · 2018Article
- Integration of Transcriptomic and Proteomic Approaches Reveals the Temperature-Dependent Virulence ofFrontiers in cellular and infection microbiology · 2018Article
- Contributions of the oligopeptide permeases in multistep of Vibrio alginolyticus pathogenesis.MicrobiologyOpen · 2017Article
- AcuC, a histone deacetylase, contributes to the pathogenicity of Aeromonas hydrophila.MicrobiologyOpen · 2017Article
- Ribose operon repressor (RbsR) contributes to the adhesion of Aeromonas hydrophila to Anguilla japonica mucus.MicrobiologyOpen · 2017Article
- flrA, flrB and flrC regulate adhesion by controlling the expression of critical virulence genes in Vibrio alginolyticus.Emerging microbes & infections · 2016Article
- The TCA Pathway is an Important Player in the Regulatory Network Governing Vibrio alginolyticus Adhesion Under Adversity.Frontiers in microbiology · 2016Article
- Involvement of the flagellar assembly pathway in Vibrio alginolyticus adhesion under environmental stresses.Frontiers in cellular and infection microbiology · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The capability of Vibrio alginolyticus to adhere to fish mucus is a key virulence factor of the bacteria. Our previous research showed that stress conditions, such as Cu(2+), Pb(2+), Hg(2+), and low pH, can reduce this adhesion ability. Non-coding (nc) RNAs play a crucial role in regulating bacterial gene expression, affecting the bacteria's pathogenicity. To investigate the mechanism(s) underlying the decline in adhesion ability caused by stressors, we combined high-throughput sequencing with computational techniques to detect stressed ncRNA dynamics. These approaches yielded three commonly altered ncRNAs that are predicted to regulate the bacterial chemotaxis pathway, which plays a key role in the adhesion process of bacteria. We hypothesized they play a key role in the adhesion process of V. alginolyticus. In this study, we validated the effects of these three ncRNAs on their predicted target genes and their role in the V. alginolyticus adhesion process with RNA interference (i), quantitative real-time polymerase chain reaction (qPCR), northern blot, capillary assay, and in vitro adhesion assays. The expression of these ncRNAs and their predicted target genes were confirmed by qPCR and northern blot, which reinforced the reliability of the sequencing data and the target prediction. Overexpression of these ncRNAs was capable of reducing the chemotactic and adhesion ability of V. alginolyticus, and the expression levels of their target genes were also significantly reduced. Our results indicated that these three ncRNAs: (1) are able to regulate the bacterial chemotaxis pathway, and (2) play a key role in the adhesion process of V. alginolyticus.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.