ArticleFrontiers in microbiology2015
Campylobacter jejuni biofilms contain extracellular DNA and are sensitive to DNase I treatment.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 84 citations in OpenAlex.
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- Pharmacological strategies for targeting biofilms in otorhinolaryngologic infections and overcoming antimicrobial resistance (Review).Biomedical reports · 2025Review
- Mechanism of recombinant 1,3-β-glucanase as antibiofilm to prevent antimicrobial resistance in antifungal therapy for vulvovaginitis candidiasis.Open veterinary journal · 2025Article
- Combined Effect of DNase I and Proteinase K on Dual-Species Biofilm ofInternational journal of food science · 2025Article
- The role ofResearch square · 2024Article
- Multitasking functions of bacterial extracellular DNA in biofilms.Journal of bacteriology · 2024Review
- Evaluation of physical and chemical isolation methods to extract and purifyFrontiers in microbiology · 2024Article
- Effect of biofilm formation in a hostile oxidative stress environment on the survival ofVeterinary world · 2024Article
- Strategies and materials for the prevention and treatment of biofilms.Materials today. Bio · 2023Review
- Extracellular matrix-degrading enzymes as a biofilm control strategy for food-related microorganisms.Food science and biotechnology · 2023Review
- Natural Antimicrobials forMicroorganisms · 2023Review
- Discovery of Novel Resistance Mechanisms ofAntibiotics (Basel, Switzerland) · 2023Article
- Relevance and Importance of Biofilms in the Resistance and Spreading of Campylobacter spp. Within the Food Chain.Advances in experimental medicine and biology · 2023Article
- The Use of Interdisciplinary Approaches to Understand the Biology ofMicroorganisms · 2022Review
- Roles of Aerotolerance, Biofilm Formation, and Viable but Non-Culturable State in the Survival ofMicroorganisms · 2022Review
- Antibiofilm effect and mechanism of protocatechuic aldehyde againstFrontiers in microbiology · 2022Article
- Antimicrobial Resistance Gene Transfer from Campylobacter jejuni in Mono- and Dual-Species Biofilms.Applied and environmental microbiology · 2021Article
- Molecular engineering of antimicrobial peptides: microbial targets, peptide motifs and translation opportunities.Biophysical reviews · 2021Review
- A novel high-content screening approach for the elucidation of C. jejuni biofilm composition and integrity.BMC microbiology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofilms make an important contribution to survival and transmission of bacterial pathogens in the food chain. The human pathogen Campylobacter jejuni is known to form biofilms in vitro in food chain-relevant conditions, but the exact roles and composition of the extracellular matrix are still not clear. Extracellular DNA has been found in many bacterial biofilms and can be a major component of the extracellular matrix. Here we show that extracellular DNA is also an important component of the C. jejuni biofilm when attached to stainless steel surfaces, in aerobic conditions and on conditioned surfaces. Degradation of extracellular DNA by exogenous addition of DNase I led to rapid biofilm removal, without loss of C. jejuni viability. Following treatment of a surface with DNase I, C. jejuni was unable to re-establish a biofilm population within 48 h. Similar results were obtained by digesting extracellular DNA with restriction enzymes, suggesting the need for high molecular weight DNA. Addition of C. jejuni genomic DNA containing an antibiotic resistance marker resulted in transfer of the antibiotic resistance marker to susceptible cells in the biofilm, presumably by natural transformation. Taken together, this suggest that eDNA is not only an important component of C. jejuni biofilms and subsequent food chain survival of C. jejuni, but may also contribute to the spread of antimicrobial resistance in C. jejuni. The degradation of extracellular DNA with enzymes such as DNase I is a rapid method to remove C. jejuni biofilms, and is likely to potentiate the activity of antimicrobial treatments and thus synergistically aid disinfection treatments.
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