ArticleFrontiers in microbiology2015
Genome mining and metabolic profiling of the rhizosphere bacterium Pseudomonas sp. SH-C52 for antimicrobial compounds.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 84 citations in OpenAlex.
- The Integrated Management of Sugar-Beet Soilborne Diseases Through Rhizosphere Microbiome Strategies: A Critical Review of Agronomic Evidence and Application Gaps.Plants (Basel, Switzerland) · 2026Review
- Higher-Level Structural Classification of Pseudomonas Cyclic Lipopeptides through Their Bioactive Conformation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Native Bacteria Are Effective Biocontrol Agents at a Wide Range of Temperatures ofPlants (Basel, Switzerland) · 2025Article
- The effects of Pseudomonas strains isolated from Achnatherum inebrians on plant growth: A genomic perspective.Environmental microbiology reports · 2024Article
- Genome Analysis ofJournal of fungi (Basel, Switzerland) · 2024Article
- Mining Biosynthetic Gene Clusters ofMicroorganisms · 2024Article
- Genome analysis of Pseudomonas strain 4B with broad antagonistic activity against toxigenic fungi.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Identification and genomic characterization ofFrontiers in microbiology · 2024Article
- Does regulation hold the key to optimizing lipopeptide production inFrontiers in bioengineering and biotechnology · 2024Review
- Nonribosomal peptides protectChemical science · 2023Article
- Uncovering Genomic Features and Biosynthetic Gene Clusters in Endophytic Bacteria from Roots of the Medicinal Plant Alkanna tinctoria Tausch as a Strategy To Identify Novel Biocontrol Bacteria.Microbiology spectrum · 2023Article
- Review
- Transporter Gene-Mediated Typing for Detection and Genome Mining of Lipopeptide-Producing Pseudomonas.Applied and environmental microbiology · 2022Article
- Review
- Genome Mining ofMolecules (Basel, Switzerland) · 2021Article
- Mitigation ofScience advances · 2021Article
- The fate of plant growth-promoting rhizobacteria in soilless agriculture: future perspectives.3 Biotech · 2021Review
- Biofertilizers and Biocontrol Agents for Agriculture: How to Identify and Develop New Potent Microbial Strains and Traits.Microorganisms · 2021Review
- Identification, Isolation, and Characterization of Medipeptins, Antimicrobial Peptides FromFrontiers in microbiology · 2021Article
- Fungal-Associated Molecules Induce Key Genes Involved in the Biosynthesis of the Antifungal Secondary Metabolites Nunamycin and Nunapeptin in the Biocontrol Strain Pseudomonas fluorescens In5.Applied and environmental microbiology · 2020Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant microbiome represents an enormous untapped resource for discovering novel genes and bioactive compounds. Previously, we isolated Pseudomonas sp. SH-C52 from the rhizosphere of sugar beet plants grown in a soil suppressive to the fungal pathogen Rhizoctonia solani and showed that its antifungal activity is, in part, attributed to the production of the chlorinated 9-amino-acid lipopeptide thanamycin (Mendes et al., 2011). To get more insight into its biosynthetic repertoire, the genome of Pseudomonas sp. SH-C52 was sequenced and subjected to in silico, mutational and functional analyses. The sequencing revealed a genome size of 6.3 Mb and 5579 predicted ORFs. Phylogenetic analysis placed strain SH-C52 within the Pseudomonas corrugata clade. In silico analysis for secondary metabolites revealed a total of six non-ribosomal peptide synthetase (NRPS) gene clusters, including the two previously described NRPS clusters for thanamycin and the 2-amino acid antibacterial lipopeptide brabantamide. Here we show that thanamycin also has activity against an array of other fungi and that brabantamide A exhibits anti-oomycete activity and affects phospholipases of the late blight pathogen Phytophthora infestans. Most notably, mass spectrometry led to the discovery of a third lipopeptide, designated thanapeptin, with a 22-amino-acid peptide moiety. Seven structural variants of thanapeptin were found with varying degrees of activity against P. infestans. Of the remaining four NRPS clusters, one was predicted to encode for yet another and unknown lipopeptide with a predicted peptide moiety of 8-amino acids. Collectively, these results show an enormous metabolic potential for Pseudomonas sp. SH-C52, with at least three structurally diverse lipopeptides, each with a different antimicrobial activity spectrum.
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