ArticleFrontiers in cellular neuroscience2015
Active mechanistic target of rapamycin plays an ancillary rather than essential role in zebrafish CNS axon regeneration.
Article in Frontiers in cellular neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Repressed mTORC1 signaling and transient dendritic pruning support axonal regeneration in adult zebrafish.iScience · 2026Article
- Serotonin neuromodulation directs optic nerve regeneration.Development (Cambridge, England) · 2025Article
- Srebf2 mediates successful optic nerve axon regeneration via the mevalonate synthesis pathway.Molecular neurodegeneration · 2025Article
- Insulin-like growth factor receptor / mTOR signaling elevates global translation to accelerate zebrafish fin regenerative outgrowth.Developmental biology · 2023Article
- Analysis of Visual Recovery After Optic Nerve Crush in Adult Zebrafish.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Review
- mTOR activity is essential for retinal pigment epithelium regeneration in zebrafish.PLoS genetics · 2022Article
- The age factor in optic nerve regeneration: Intrinsic and extrinsic barriers hinder successful recovery in the short-living killifish.Aging cell · 2022Article
- Differential Retinal Ganglion Cell Vulnerability, A Critical Clue for the Identification of Neuroprotective Genes in Glaucoma.Frontiers in ophthalmology · 2022Review
- Cellular reprogramming for successful CNS axon regeneration is driven by a temporally changing cast of transcription factors.Scientific reports · 2019Article
- An Antagonistic Axon-Dendrite Interplay Enables Efficient Neuronal Repair in the Adult Zebrafish Central Nervous System.Molecular neurobiology · 2019Article
- Modest enhancement of sensory axon regeneration in the sciatic nerve with conditional co-deletion of PTEN and SOCS3 in the dorsal root ganglia of adult mice.Experimental neurology · 2018Article
- Axonal regeneration in zebrafish spinal cord.Regeneration (Oxford, England) · 2018Review
- Article
- Translational profiling of retinal ganglion cell optic nerve regeneration in Xenopus laevis.Developmental biology · 2017Article
- Neuroinflammation as Fuel for Axonal Regeneration in the Injured Vertebrate Central Nervous System.Mediators of inflammation · 2017Review
- Highly efficient transduction of primary adult CNS and PNS neurons.Scientific reports · 2016Article
- Understanding the Role of ztor in Aging-related Diseases Using the Zebrafish Model.In vivo (Athens, Greece)Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The developmental decrease of the intrinsic regenerative ability of the mammalian central nervous system (CNS) is associated with reduced activity of mechanistic target of rapamycin (mTOR) in mature neurons such as retinal ganglion cells (RGCs). While mTOR activity is further decreased upon axonal injury, maintenance of its pre-injury level, for instance by genetic deletion of the phosphatase and tensin homolog (PTEN), markedly promotes axon regeneration in mammals. The current study now addressed the question whether active mTOR might generally play a central role in axon regeneration by analyzing its requirement in regeneration-competent zebrafish. Remarkably, regulation of mTOR activity after optic nerve injury in zebrafish is fundamentally different compared to mammals. Hardly any activity was detected in naïve RGCs, whereas it was markedly increased upon axotomy in vivo as well as in dissociated cell cultures. After a short burst, mTOR activity was quickly attenuated, which is contrary to the requirements for axon regeneration in mammals. Surprisingly, mTOR activity was not essential for axonal growth per se, but correlated with cytokine- and PTEN inhibitor-induced neurite extension in vitro. Moreover, inhibition of mTOR using rapamycin significantly reduced axon regeneration in vivo and compromised functional recovery after optic nerve injury. Therefore, axotomy-induced mTOR activity is involved in CNS axon regeneration in zebrafish similar to mammals, although it plays an ancillary rather than essential role in this regeneration-competent species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.