Evidence map›Paper›PMID 26217104›Full record

ArticleGenomics insights2011

Cloning and Initial Functional Characterization of Mlk4α and Mlk4β.

Vladimir I Kashuba, Elvira V Grigorieva, Sergei M Kvasha, Tatiana V Pavlova, Vladimir Grigoriev, Alexei Protopopov, Olga Kharchenko, Rinat Gizatullin, Alla V Rynditch, Eugene R Zabarovsky

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genomics insights, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.3field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 4 countries.

Vladimir I KashubaDepartment of Microbiology, Tumor and Cell Biology, Department of Clinical Science and Education, Södersjukhuset, Karolinska Institute, Stockholm, 17177, Sweden. ; Institute of Molecular Biology and Genetics, Ukrainian National Academy of Sciences, Kiev, 03143, Ukraine.
Elvira V GrigorievaDepartment of Microbiology, Tumor and Cell Biology, Department of Clinical Science and Education, Södersjukhuset, Karolinska Institute, Stockholm, 17177, Sweden. ; Institute of Molecular Biology and Biophysics, Siberian Division of Russian Academy of Medical Sciences, Novosibirsk, 630117, Russia.
Sergei M KvashaInstitute of Molecular Biology and Genetics, Ukrainian National Academy of Sciences, Kiev, 03143, Ukraine.
Tatiana V PavlovaDepartment of Microbiology, Tumor and Cell Biology, Department of Clinical Science and Education, Södersjukhuset, Karolinska Institute, Stockholm, 17177, Sweden. ; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
Vladimir GrigorievInstitute of Cancer Research, 237 Fulham Road, London SW3 6JB, UK.
Alexei ProtopopovDepartment of Microbiology, Tumor and Cell Biology, Department of Clinical Science and Education, Södersjukhuset, Karolinska Institute, Stockholm, 17177, Sweden.
Olga KharchenkoDepartment of Microbiology, Tumor and Cell Biology, Department of Clinical Science and Education, Södersjukhuset, Karolinska Institute, Stockholm, 17177, Sweden. ; Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, 199034, Russia.
Rinat GizatullinDepartment of Microbiology, Tumor and Cell Biology, Department of Clinical Science and Education, Södersjukhuset, Karolinska Institute, Stockholm, 17177, Sweden.
Alla V RynditchInstitute of Molecular Biology and Genetics, Ukrainian National Academy of Sciences, Kiev, 03143, Ukraine.
Eugene R ZabarovskyDepartment of Microbiology, Tumor and Cell Biology, Department of Clinical Science and Education, Södersjukhuset, Karolinska Institute, Stockholm, 17177, Sweden. ; Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia.
Karolinska Institutet · SEEngelhardt Institute of Molecular Biology · RUInstitute of Molecular Biology and Genetics · UAInstitute of Cancer Research · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have cloned a novel human mixed-lineage kinase gene, MLK4. Two alternatively spliced forms, MLK4α (580 aa) and MLK4β (1036 aa), have been identified and mapped to chromosomal band 1q42. MLK4 shows high amino acid homology to the kinase catalytic domain of MLK3 (72%), MLK1 (71%) and MLK2 (69%). Strong expression of MLK4 was detected in the human pancreas and kidneys. pCMV-MLK4β c-myc-tagged protein (human) was expressed in the cytoplasm and nucleus of transiently transfected COS-1 cells, while pCMV-MLK4α c-myc-tagged protein (human) was expressed in cytoplasm only. Both MLK4 isoforms reduced the colony formation ability of MCF7 cells by 85%-95% and almost totally suppressed cell proliferation in the CyQUANT cell proliferation assay. Human pCMV-MLK4β transgenic mice expressed the MLK4β in all tissues examined but no phenotypic abnormalities were observed. Thus, in this work, we present the cloning and sequencing of MLK4α and MLK4β for the first time; the data obtained suggest that MLK4 may function as a MAP kinase.

Indexed as

gene cloningMLKprotein kinasetumor suppressor gene

Identifiers

PMID26217104
PMCPMC4510602
OpenAlexW2075988157

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.