ArticleProceedings of the National Academy of Sciences of the United States of America2015
Two transcription pause elements underlie a σ70-dependent pause cycle.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Bacteroides expand the functional versatility of a conserved transcription factor and transcribed DNA to program capsule diversity.Nature communications · 2024Article
- The yin and yang of the universal transcription factor NusG.Current opinion in microbiology · 2024Review
- Structural and functional basis of the universal transcription factor NusG pro-pausing activity in Mycobacterium tuberculosis.Molecular cell · 2023Article
- An ensemble of interconverting conformations of the elemental paused transcription complex creates regulatory options.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Studying RNA Polymerase II Promoter-Proximal Pausing by In Vitro Immobilized Template and Transcription Assays.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Structural basis of AlpA-dependent transcription antitermination.Nucleic acids research · 2022Article
- Structural and mechanistic basis of σ-dependent transcriptional pausing.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Nascent RNA sequencing identifies a widespread sigma70-dependent pausing regulated by Gre factors in bacteria.Nature communications · 2021Article
- Chemical roadblocking of DNA transcription for nascent RNA display.The Journal of biological chemistry · 2020Article
- A ligand-gated strand displacement mechanism for ZTP riboswitch transcription control.Nature chemical biology · 2019Article
- Structural basis of Q-dependent antitermination.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Structural basis of Q-dependent transcription antitermination.Nature communications · 2019Article
- Article
- Article
- Review
- Transcription elongation.Transcription · 2017Review
- Article
- σ38-dependent promoter-proximal pausing by bacterial RNA polymerase.Nucleic acids research · 2017Article
- A structure-based kinetic model of transcription.Transcription · 2017Article
- RNA polymerase supply and flux through the lac operon in Escherichia coli.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The movement of RNA polymerase (RNAP) during transcription elongation is modulated by DNA-encoded elements that cause the elongation complex to pause. One of the best-characterized pause sequences is a binding site for the σ(70) initiation factor that induces pausing at a site near lambdoid phage late-gene promoters. An essential component of this σ(70)-dependent pause is the elemental pause site (EPS), a sequence that itself induces transcription pausing throughout the Escherichia coli genome and underlies other complex regulatory pause elements, such as the ops and his operon pauses. Here, we identify and provide a detailed kinetic analysis of a transcription cycle analogous to abortive cycling that underlies the σ(70)-dependent pause. We show that, in σ(70)-dependent pausing, the elemental pause acts primarily to modulate the rate at which complexes attempt to disengage the σ(70):DNA interaction. Our findings establish the σ(70)-dependent pause-encoding region as a multipartite element in which several pause-inducing components make distinct mechanistic contributions to the induction and maintenance of a regulatory transcription pause.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.