ArticleProceedings of the National Academy of Sciences of the United States of America2015
Single-cell measurement of red blood cell oxygen affinity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03101215 (Workload of Water Polo Players Following a Phosphorus Manipulated High Carbohydrate Meal), which is not on this map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Workload of Water Polo Players Following a Phosphorus Manipulated High Carbohydrate Meal
Who cites it
29 citing papers in PubMed, 46 citations in OpenAlex.
- Microfluidics for Blood Disorders and Hematological Disease Monitoring and Modeling.International journal of molecular sciences · 2026Review
- Quantifying the unique mechanical properties of irreversibly sickled cells in sickle cell disease.Blood vessels, thrombosis & hemostasis · 2025Article
- Novel single-cell measurements suggest irreversibly sickled cells are neither dense nor dehydrated.Biophysical journal · 2024Article
- The volume of healthy red blood cells is optimal for advective oxygen transport in arterioles.Biophysical journal · 2024Article
- Rapid measurement of hemoglobin-oxygen dissociation by leveraging Bohr effect and Soret band bathochromic shift.The Analyst · 2024Article
- High-throughput quantification of red blood cell deformability and oxygen saturation to probe mechanisms of sickle cell disease.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Dual-view transport of intensity phase imaging flow cytometry.Biomedical optics express · 2023Article
- Simultaneous quantification of blood rheology and oxygen saturation to evaluate affinity-modifying therapies in sickle cell disease.Lab on a chip · 2022Article
- Phenotypic screening of the ReFRAME drug repurposing library to discover new drugs for treating sickle cell disease.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Fluorescence Lifetime Measurement of Prefibrillar Sickle Hemoglobin Oligomers as a Platform for Drug Discovery in Sickle Cell Disease.Biomacromolecules · 2022Article
- Specificity of Nuclear Size Scaling in Frog Erythrocytes.Frontiers in cell and developmental biology · 2022Article
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- Network-driven anomalous transport is a fundamental component of brain microvascular dysfunction.Nature communications · 2021Article
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- Remote ischemic conditioning enhances oxygen supply to ischemic brain tissue in a mouse model of stroke: Role of elevated 2,3-biphosphoglycerate in erythrocytes.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2021Article
- Assessment of transient changes in oxygen diffusion of single red blood cells using a microfluidic analytical platform.Communications biology · 2021Article
- Development of Porous and Flexible PTMC Membranes for In Vitro Organ Models Fabricated by Evaporation-Induced Phase Separation.Membranes · 2020Article
- Potential attenuation of biochemical parameters and enzymatic functions in Cyprinus carpio fingerlings by Phenthoate 50 EC insecticide exposure.Environmental science and pollution research international · 2020Article
- Spectroscopic Evaluation of Red Blood Cells of Thalassemia Patients with Confocal Microscopy: A Pilot Study.Sensors (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Oxygen is transported throughout the body by hemoglobin (Hb) in red blood cells (RBCs). Although the oxygen affinity of blood is well-understood and routinely assessed in patients by pulse oximetry, variability at the single-cell level has not been previously measured. In contrast, single-cell measurements of RBC volume and Hb concentration are taken millions of times per day by clinical hematology analyzers, and they are important factors in determining the health of the hematologic system. To better understand the variability and determinants of oxygen affinity on a cellular level, we have developed a system that quantifies the oxygen saturation, cell volume, and Hb concentration for individual RBCs in high throughput. We find that the variability in single-cell saturation peaks at an oxygen partial pressure of 2.9%, which corresponds to the maximum slope of the oxygen-Hb dissociation curve. In addition, single-cell oxygen affinity is positively correlated with Hb concentration but independent of osmolarity, which suggests variation in the Hb to 2,3-diphosphoglycerate (2-3 DPG) ratio on a cellular level. By quantifying the functional behavior of a cellular population, our system adds a dimension to blood cell analysis and other measurements of single-cell variability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.