ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2015
A Historical Perspective on the Identification of Cell Types in Pancreatic Islets of Langerhans by Staining and Histochemical Techniques.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Islet ultrastructure: past achievements and future directions.Diabetologia · 2026Review
- Paul Langerhans (1847-1888): perceiving the unknown and describing it.Pathologica · 2025Article
- Engineered tools to study endocrine dysfunction of pancreas.Biophysics reviews · 2024Review
- Pancreatic Crosstalk in the Disease Setting: Understanding the Impact of Exocrine Disease on Endocrine Function.Comprehensive Physiology · 2024Review
- Protective potential of fish oil supplementation against insulin resistance and pancreatic islet damage in STZ-induced Wistar rats.Open veterinary journal · 2023Article
- Review
- Feline Adipose Derived Multipotent Stromal Cell Transdifferentiation Into Functional Insulin Producing Cell Clusters.Frontiers in bioengineering and biotechnology · 2022Article
- Somatostatin and Its Receptor System in Colorectal Cancer.Biomedicines · 2021Review
- Article
- Effects of sustained GABA releasing implants on pancreatic islets in mice.Drug delivery and translational research · 2021Article
- The Human Islet: Mini-Organ With Mega-Impact.Endocrine reviews · 2021Review
- A pilot study of estrogen receptor (ER) expression in pancreatic ductal adenocarcinoma (PDAC).Translational gastroenterology and hepatology · 2021Article
- Review
- In Vivo Studies of Inoculated Plants and In Vitro Studies Utilizing Methanolic Extracts of EndophyticInternational journal of molecular sciences · 2020Article
- An imazamox-based herbicide causes apoptotic changes in rat liver and pancreas.Toxicology reports · 2019Article
- The Difference δ-Cells Make in Glucose Control.Physiology (Bethesda, Md.) · 2018Review
- The somatostatin-secreting pancreatic δ-cell in health and disease.Nature reviews. Endocrinology · 2018Review
- Role of extracellular matrix in the biomechanical behavior of pancreatic tissue.ACS biomaterials science & engineering · 2018Article
- Organ donor pancreases for the study of human islet cell histology and pathophysiology: a precious and valuable resource.Diabetologia · 2018Article
- Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio.PloS one · 2017Article
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Abstract
Before the middle of the previous century, cell types of the pancreatic islets of Langerhans were identified primarily on the basis of their color reactions with histological dyes. At that time, the chemical basis for the staining properties of islet cells in relation to the identity, chemistry and structure of their hormones was not fully understood. Nevertheless, the definitive islet cell types that secrete glucagon, insulin, and somatostatin (A, B, and D cells, respectively) could reliably be differentiated from each other with staining protocols that involved variations of one or more tinctorial techniques, such as the Mallory-Heidenhain azan trichrome, chromium hematoxylin and phloxine, aldehyde fuchsin, and silver impregnation methods, which were popularly used until supplanted by immunohistochemical techniques. Before antibody-based staining methods, the most bona fide histochemical techniques for the identification of islet B cells were based on the detection of sulfhydryl and disulfide groups of insulin. The application of the classical islet tinctorial staining methods for pathophysiological studies and physiological experiments was fundamental to our understanding of islet architecture and the physiological roles of A and B cells in glucose regulation and diabetes.
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