Evidence map›Paper›PMID 26215316›Full record

ArticleJournal of periodontal research2016

Gene-expression analysis of cementoblasts and osteoblasts.

B G Matthews, H Roguljic, T Franceschetti, E Roeder, I Matic, I Vidovic, P Joshi, K-Y Kum, I Kalajzic

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of periodontal research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 46 citations in OpenAlex.

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  15. Adiponectin as Well as Compressive Forces RegulateFrontiers in cell and developmental biology · 2021
    Article
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  18. Transcriptomics: a Solution for Renal Osteodystrophy?Current osteoporosis reports · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

B G MatthewsDepartment of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, Farmington, CT, USA.
H RoguljicDepartment of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, Farmington, CT, USA.
T FranceschettiDepartment of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, Farmington, CT, USA.
E RoederDepartment of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, Farmington, CT, USA.
I MaticDepartment of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, Farmington, CT, USA.
I VidovicDivision of Pediatric Dentistry, Department of Craniofacial Sciences, School of Dental Medicine, University of Connecticut Health Center, Farmington, CT, USA.
P JoshiDepartment of Computer Science and Engineering, University of Connecticut, Storrs, CT, USA.
K-Y KumDivision of endodontology, Department of Oral Health and Diagnostic Sciences, School of Dental Medicine, University of Connecticut Health Center, Farmington, CT, USA.
I KalajzicDepartment of Reconstructive Sciences, School of Dental Medicine, University of Connecticut Health Center, Farmington, CT, USA.
UConn Health · USIn-Q-Tel · USSeoul National University Dental Hospital · KRUniversity of Connecticut · US

Funding

Notch Signaling and Bone Fracture HealingR01AR055607 · NIAMS · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI Kurt David Hankenson, IVO Kalajzic · 2011 to 2026
$6.3M
Skeletal, Craniofacial and Oral Biology Training GrantR90DE022526 · NIDCR · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI MINA, MINA · 2011 to 2022
$627k
NIAMS NIH HHS R01 AR055607NIDCR NIH HHS R90 DE022526
6 · The paper itself

Abstract

BACKGROUND AND

objectiveCementum and bone are similar mineralized tissues, but cementum accumulates much more slowly than bone, does not have vasculature or innervation and does not undergo remodeling. Despite these differences, there are no well-established markers to distinguish cementoblasts from other mature mineralizing cells such as osteoblasts and odontoblasts. The purpose of this study was to assess differences in gene expression between cementoblasts and osteoblasts using gene profiling of cell populations isolated directly from osteocalcin-green fluorescent protein (OC-GFP) transgenic mice. MATERIAL AND

methodsOC-GFP reporter mice were used as they show labeling of cementoblasts, osteoblasts and odontoblasts, but not of periodontal ligament fibroblasts, within the periodontium. We sorted cells digested from the molar root surface to isolate OC-GFP(+) cementoblasts. Osteoblasts were isolated from calvarial digests. Microarray analysis was performed, and selected results were confirmed by real-time PCR and immunostaining or in situ hybridization.

resultsMicroarray analysis identified 95 genes that were expressed at least two-fold higher in cementoblasts than in osteoblasts. Our analysis indicated that the Wnt signaling pathway was differentially regulated, as were genes related to skeletal development. Real-time PCR confirmed that expression of the Wnt inhibitors Wnt inhibitory factor 1 (Wif1) and secreted frizzled-related protein 1 (Sfrp1) was elevated in cementoblasts compared with osteoblasts, and Wif1 expression was localized to the apical root region. In addition, the transcription factor BARX homeobox 1 (Barx1) was expressed at higher levels in cementoblasts, and immunohistochemistry indicated that BARX1 was expressed in apical cementoblasts and cementocytes, but not in osteoblasts or odontoblasts.

conclusionThe OC-GFP mouse provides a good model for selectively isolating cementoblasts, and allowed for identification of differentially expressed genes between cementoblasts and osteoblasts.

Indexed as

Gene Expression RegulationAdaptor Proteins, Signal TransducingAnimalsCalcification, PhysiologicCell DifferentiationDental CementumExtracellular Matrix ProteinsFibroblastsHomeodomain ProteinsIntercellular Signaling Peptides and ProteinsMembrane ProteinsMiceMice, TransgenicOdontoblastsOsteoblastsOsteocalcinAdaptor Proteins, Signal TransducingBarx1 protein, mouseExtracellular Matrix ProteinsHomeodomain ProteinsIntercellular Signaling Peptides and ProteinsMembrane ProteinsOsteocalcinRNA, MessengerSecreted Frizzled-Related ProteinsSfrp1 protein, mouseTranscription FactorsWif1 protein, mousecementoblastgene expressionosteoblastosteocalcinWnt signaling pathway

Identifiers

PMID26215316
PMCPMC4729669
OpenAlexW1549178231

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.