ArticlePlant biotechnology journal2016
Enhanced resistance in Theobroma cacao against oomycete and fungal pathogens by secretion of phosphatidylinositol-3-phosphate-binding proteins.
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 24 citations in OpenAlex.
- Interactions of Different Species ofMicroorganisms · 2023Review
- Engineering crop Phytophthora resistance by targeting pathogen-derived PI3P for enhanced catabolism.Plant communications · 2023Article
- DNA barcoding and comparative RNA-Seq analysis provide new insights into leaf formation using a novel resource of high-yieldingFrontiers in plant science · 2023Article
- Transgenic Soybeans Expressing Phosphatidylinositol-3-Phosphate-Binding Proteins Show Enhanced Resistance Against the Oomycete PathogenFrontiers in microbiology · 2022Article
- Tethering of Multi-Vesicular Bodies and the Tonoplast to the Plasma Membrane in Plants.Frontiers in plant science · 2019Article
- Recruitment of Vps34 PI3K and enrichment of PI3P phosphoinositide in the viral replication compartment is crucial for replication of a positive-strand RNA virus.PLoS pathogens · 2019Article
- Disruption of OsSEC3A increases the content of salicylic acid and induces plant defense responses in rice.Journal of experimental botany · 2018Article
- Glucocorticoid receptor-regulated TcLEC2 expression triggers somatic embryogenesis in Theobroma cacao leaf tissue.PloS one · 2018Article
- Transient Expression of CRISPR/Cas9 Machinery TargetingFrontiers in plant science · 2018Article
- Protocol: transient expression system for functional genomics in the tropical tree Theobroma cacao L.Plant methods · 2016Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The internalization of some oomycete and fungal pathogen effectors into host plant cells has been reported to be blocked by proteins that bind to the effectors' cell entry receptor, phosphatidylinositol-3-phosphate (PI3P). This finding suggested a novel strategy for disease control by engineering plants to secrete PI3P-binding proteins. In this study, we tested this strategy using the chocolate tree Theobroma cacao. Transient expression and secretion of four different PI3P-binding proteins in detached leaves of T. cacao greatly reduced infection by two oomycete pathogens, Phytophthora tropicalis and Phytophthora palmivora, which cause black pod disease. Lesion size and pathogen growth were reduced by up to 85%. Resistance was not conferred by proteins lacking a secretory leader, by proteins with mutations in their PI3P-binding site, or by a secreted PI4P-binding protein. Stably transformed, transgenic T. cacao plants expressing two different PI3P-binding proteins showed substantially enhanced resistance to both P. tropicalis and P. palmivora, as well as to the fungal pathogen Colletotrichum theobromicola. These results demonstrate that secretion of PI3P-binding proteins is an effective way to increase disease resistance in T. cacao, and potentially in other plants, against a broad spectrum of pathogens.
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