ArticleAutonomic neuroscience : basic & clinical2015
Isoflurane causes concentration-dependent inhibition of medullary raphé 5-HT neurons in situ.
Article in Autonomic neuroscience : basic & clinical, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Acute psilocin increased cortical activity in rat.Frontiers in neuroscience · 2026Article
- Acute psilocybin increased cortical activities in rats.Frontiers in neuroscience · 2023Article
- Analyzing the brainstem circuits for respiratory chemosensitivity in freely moving mice.eLife · 2022Article
- Serotonin Transporter and Plasma Membrane Monoamine Transporter Are Necessary for the Antidepressant-Like Effects of Ketamine in Mice.International journal of molecular sciences · 2020Article
- Volatile Anesthetics Activate a Leak Sodium Conductance in Retrotrapezoid Nucleus Neurons to Maintain Breathing during Anesthesia in Mice.Anesthesiology · 2020Article
- Type of Anaesthetic Influences [Molecular imaging and biology · 2020Article
- Towards a better understanding of anesthesia emergence mechanisms: Research and clinical implications.World journal of methodology · 2018Review
- Serotonin Decreases the Gain of Visual Responses in Awake Macaque V1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2017Article
- Isoflurane, ketamine-xylazine, and urethane markedly alter breathing even at subtherapeutic doses.Journal of neurophysiology · 2017Article
- Marmoset Serotonin 5-HT1A Receptor Mapping with a Biased Agonist PET Probe 18F-F13714: Comparison with an Antagonist Tracer 18F-MPPF in Awake and Anesthetized States.The international journal of neuropsychopharmacology · 2016Article
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5 authors at 1 institution in 1 country.
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Abstract
backgroundAnesthetics have a profound influence on a myriad of autonomic processes. Mechanisms of general anesthesia, and how these mechanisms give rise to the multifaceted state of anesthesia, are largely unknown. The ascending and descending serotonin (5-HT) networks are key modulators of autonomic pathways, and are critically involved in homeostatic reflexes across the motor, somatosensory, limbic and autonomic systems. These 5-HT networks are thought to contribute to anesthetic effects, but how anesthetics affect 5-HT neuron function remains a pertinent question. We hypothesized that the volatile anesthetic isoflurane inhibits action potential discharge of medullary raphé 5-HT neurons.
methodsWe conducted extracellular recordings on individual neurons in the medullary raphé region of the unanesthetized in situ perfused brainstem preparation to determine how exposure to isoflurane affects 5-HT neurons. We examined changes in 5-HT neuron baseline firing in response to treatment with either 1, 1.5, or 2% isoflurane. We measured isoflurane concentrations by gas chromatography-mass spectrometry (GC-MS) analysis.
resultsExposure to isoflurane inhibited action potential discharge in raphé 5-HT neurons. We document a concentration-dependent inhibition over a range of concentrations approximating isoflurane MAC (minimum alveolar concentration required for surgical anesthesia). Delivered concentrations of isoflurane were confirmed using GC-MS analysis.
conclusionsThese findings illustrate that halogenated anesthetics greatly affect 5-HT neuron firing and suggest 5-HT neuron contributions to mechanisms of general anesthesia.
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