ArticleEpigenetics2015
Q-RRBS: a quantitative reduced representation bisulfite sequencing method for single-cell methylome analyses.
Article in Epigenetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Single-cell omics: experimental workflow, data analyses and applications.Science China. Life sciences · 2025Review
- Advances in single-cell omics and multiomics for high-resolution molecular profiling.Experimental & molecular medicine · 2024Review
- Identification and validation of a DNA methylation-driven gene-based prognostic model for clear cell renal cell carcinoma.BMC genomics · 2023Article
- Technologies and applications of single-cell DNA methylation sequencing.Theranostics · 2023Review
- Single-Cell DNA Methylation Analysis in Cancer.Cancers · 2022Review
- Review
- Current and Emerging Technologies for the Analysis of the Genome-Wide and Locus-Specific DNA Methylation Patterns.Advances in experimental medicine and biology · 2022Article
- Introduction to Single-Cell DNA Methylation Profiling Methods.Biomolecules · 2021Review
- Application of single cell genomics to focal epilepsies: A call to action.Brain pathology (Zurich, Switzerland) · 2021Review
- Somatic mutations in colorectal cancer are associated with the epigenetic modifications.Journal of cellular and molecular medicine · 2020Article
- Mapping chromatin modifications at the single cell level.Development (Cambridge, England) · 2019Review
- Comprehensive Evaluation of Commercial Bisulfite-Based DNA Methylation Kits and Development of an Alternative Protocol With Improved Conversion Performance.Genetics & epigenetics · 2018Article
- Bisulfite-independent analysis of CpG island methylation enables genome-scale stratification of single cells.Nucleic acids research · 2017Article
- Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress.International journal of biological sciences · 2017Article
- Base-resolution profiling of active DNA demethylation using MAB-seq and caMAB-seq.Nature protocols · 2016Article
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Authors and funding
10 authors.
Funding
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Abstract
Reduced representation bisulfite sequencing (RRBS) is a powerful method of DNA methylome profiling that can be applied to single cells. However, no previous report has described how PCR-based duplication-induced artifacts affect the accuracy of this method when measuring DNA methylation levels. For quantifying the effects of duplication-induced artifacts on methylome profiling when using ultra-trace amounts of starting material, we developed a novel method, namely quantitative RRBS (Q-RRBS), in which PCR-induced duplication is excluded through the use of unique molecular identifiers (UMIs). By performing Q-RRBS on varying amounts of starting material, we determined that duplication-induced artifacts were more severe when small quantities of the starting material were used. However, through using the UMIs, we successfully eliminated these artifacts. In addition, Q-RRBS could accurately detect allele-specific methylation in absence of allele-specific genetic variants. Our results demonstrate that Q-RRBS is an optimal strategy for DNA methylation profiling of single cells or samples containing ultra-trace amounts of cells.
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