Evidence map›Paper›PMID 26212728›Full record

ArticlePlasmid2015

The Xis2d protein of CTnDOT binds to the intergenic region between the mob and tra operons.

Crystal M Hopp, Jeffrey F Gardner, Abigail A Salyers

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plasmid, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Crystal M HoppDepartment of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA. Electronic address: chopp2@illinois.edu.
Jeffrey F GardnerDepartment of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Abigail A SalyersDepartment of Microbiology, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
University of Illinois Urbana-Champaign · US

Funding

SITE-SPECIFIC RECOMBINATIONR01GM028717 · NIGMS · UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN · PI GARDNER, JEFFREY F · 1985 to 2013
$4.0M
NETWORK TO MONITOR RESISTANCE IN COMMENSAL BACTERIAR01AI022383 · NIAID · UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN · PI SALYERS, ABIGAIL A · 1985 to 2010
$3.0M
Conjugal Transfer of Bacteriodes Antibiotic ResistancesR56AI022383 · NIAID · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI GARDNER, JEFFREY F · 2011 to 2011
$359k
NIAID NIH HHS AI 22383NIAID NIH HHS R01 AI022383NIAID NIH HHS R56 AI022383NIGMS NIH HHS GM 28717NIGMS NIH HHS R01 GM028717
6 · The paper itself

Abstract

CTnDOT is a 65kbp integrative and conjugative element (ICE) that carries genes encoding both tetracycline and erythromycin resistances. The excision operon of this element encodes Xis2c, Xis2d, and Exc proteins involved in the excision of CTnDOT from host chromosomes. These proteins are also required in the complex transcriptional regulation of the divergently transcribed transfer (tra) and mobilization (mob) operons of CTnDOT. Transcription of the tra operon is positively regulated by Xis2c and Xis2d, whereas, transcription of the mob operon is positively regulated by Xis2d and Exc. Xis2d is the only protein that is involved in the excision reaction, as well as the transcriptional regulation of both the mob and tra operons. This paper helps establish how Xis2d binds the DNA in the mob and tra region. Unlike other excisionase proteins, Xis2d binds a region of dyad symmetry. The binding site is located in the intergenic region between the mob and tra promoters, and once bound Xis2d induces a bend in the DNA. Xis2d binding to this region could be the preliminary step for the activation of both operons. Then the other proteins, like Exc, can interact with Xis2d and form higher order complexes.

Indexed as

Conjugation, GeneticDNA, IntergenicDNA Transposable ElementsOperonBacterial ProteinsBase SequenceGene OrderGenetic LociMolecular Sequence DataMutationPlasmidsProtein BindingBacterial ProteinsDNA, IntergenicDNA Transposable ElementsConjugative transposonIntegrative and conjugative elementRecombination directionality factor

Identifiers

PMID26212728
PMCPMC4600444
OpenAlexW958335321

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.