ArticleScientific reports2015
Characterization of RNA silencing components in the plant pathogenic fungus Fusarium graminearum.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 136 citations in OpenAlex.
- A Viral RNA Silencing Suppressor Modulates Reactive Oxygen Species Levels to Induce the Autophagic Degradation of Dicer-Like and Argonaute-Like Proteins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Tandem repeat-induced sexual silencing: A Rid-dependent RNAi mechanism for fungal genome defense via translational repression.Science advances · 2025Article
- Methylation of mycovirus DNA is mediated by the RNAi machinery in vegetative hyphae of Fusarium graminearum.Nucleic acids research · 2025Article
- Trans-Kingdom RNA Dialogues: miRNA and milRNA Networks as Biotechnological Tools for Sustainable Crop Defense and Pathogen Control.Plants (Basel, Switzerland) · 2025Review
- Article
- Review
- Harnessing RNA interference for the control of Fusarium species: A critical review.Molecular plant pathology · 2024Review
- RNAi-Based Approaches to Control Mycotoxin Producers: Challenges and Perspectives.Journal of fungi (Basel, Switzerland) · 2024Review
- Challenges and Opportunities Arising from Host-International journal of molecular sciences · 2024Review
- Article
- The RNAi Machinery in the FungusNon-coding RNA · 2024Article
- A fungal RNA-dependent RNA polymerase is a novel player in plant infection and cross-kingdom RNA interference.PLoS pathogens · 2023Article
- Genome-wide identification of Sclerotinia sclerotiorum small RNAs and their endogenous targets.BMC genomics · 2023Article
- A Mycovirus VIGS Vector Confers Hypovirulence to a Plant Pathogenic Fungus to Control Wheat FHB.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- RNA-Based Control of Fungal Pathogens in Plants.International journal of molecular sciences · 2023Review
- Disruption of theRNA (New York, N.Y.) · 2023Article
- Exogenous double-stranded RNA inhibits the infection physiology of rust fungi to reduce symptoms in planta.Molecular plant pathology · 2023Article
- A beneficial fungal root endophyte triggers systemic RNA silencing and DNA methylation of a host reporter gene.RNA biology · 2023Article
- Interference of Small RNAs inJournal of fungi (Basel, Switzerland) · 2022Article
- Small RNA Analyses of aViruses · 2022Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The RNA interference (RNAi) plays a critical role in gene regulation in a variety of eukaryotic organisms. However, the role of RNAi remains largely unclear in plant pathogenic fungi. In this study, we explored the roles of core components of the RNAi pathway in Fusarium graminearum, the major causal agent of wheat head blight. Our results demonstrated that the hairpin RNA (hpRNA) can efficiently silence the expression level of target gene, and the argonaute protein FgAgo1 and dicer protein FgDicer2 are important in this silencing process. RNAi machinery was not involved in growth, abiotic stress and pathogenesis in F. graminearum under tested conditions. We firstly applied high-throughput sequencing technology to elucidate small RNA (17-40 nucleotides) (sRNA) transcriptome in F. graminearum, and found that a total of forty-nine micro-like-RNA (milRNA) candidates were identified in the wild-type and ∆FgDICER2, and twenty-four of them were FgDicer2-dependent. Fg-milRNA-4 negatively regulated expression of its target gene. Taken together, our results indicated that the hpRNA-induced gene silencing was a valuable genetic tool for exploring gene function in F. graminearum. FgAgo1 and FgDicer2 proteins played a critical role in the hpRNA mediated gene silencing process. In addition, FgDicer2 was involved in sRNA transcription and milRNA generation in this fungus.
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