ArticleParasitology research2015
Development of a rapid HRM genotyping method for detection of dog-derived Giardia lamblia.
Article in Parasitology research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Evolution ofJournal of parasitology research · 2026Review
- High resolution melting real-time PCR for genotyping of Giardia lamblia assemblages A and B regardless of parasite load.Gut pathogens · 2025Article
- Establishment and preliminary application of PCR-RFLP genotyping method for Giardia duodenalis in goats.BMC veterinary research · 2024Article
- Rapid on-site diagnosis of canine giardiosis: time versus performance.Parasites & vectors · 2020Article
- Development of TParasitology research · 2017Article
- PCR-RFLP method to detect zoonotic and host-specific Giardia duodenalis assemblages in dog fecal samples.Parasitology research · 2016Article
- Genotype Characteristics ofIranian journal of parasitologyArticle
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Giardia lamblia is a zoonotic flagellate protozoan in the intestine of human and many mammals including dogs. To assess a threat of dog-derived G. lamblia to humans, the common dog-derived G. lamblia assemblages A, C, and D were genotyped by high-resolution melting (HRM) technology. According to β-giardin gene sequence, the qPCR-HRM primers BG5 and BG7 were designed. A series of experiments on the stability, sensitivity, and accuracy of the HRM method were also tested. Results showed that the primers BG5 and BG7 could distinguish among three assemblages A, C, and D, which Tm value differences were about 1 °C to each other. The melting curves of intra-assay reproducibility were almost coincided, and those of inter-assay reproducibility were much the same shape. The lowest detection concentration was about 5 × 10(-6)-ng/μL sample. The genotyping results from 21 G. lamblia samples by the HRM method were in complete accordance with sequencing results. It is concluded that the HRM genotyping method is rapid, stable, specific, highly sensitive, and suitable for clinical detection and molecular epidemiological survey of dog-derived G. lamblia.
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