ArticleProtoplasma2016
De novo assembly and characterization of global transcriptome of coconut palm (Cocos nucifera L.) embryogenic calli using Illumina paired-end sequencing.
Article in Protoplasma, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 75 citations in OpenAlex.
- Molecular insights of somatic embryogenesis in coconut.Molecular biology reports · 2025Review
- Conserved stress-responsive genes involved in the early development of Euterpe Edulis.Scientific reports · 2025Article
- Chromosome scale genome assembly and annotation of coconut cultivar Chowghat Green Dwarf.Scientific reports · 2024Article
- Genome-wide identification, characterization, and expression analysis of the small auxin-up RNA gene family during zygotic and somatic embryo maturation of the cacao tree (Theobroma cacao).Genomics & informatics · 2024Article
- Article
- Comparative transcriptome analysis of high- and low-embryogenic Hevea brasiliensis genotypes reveals involvement of phytohormones in somatic embryogenesis.BMC plant biology · 2023Article
- Genomics and Transcriptomics Reveal Genetic Contribution to Population Diversity and Specific Traits in Coconut.Plants (Basel, Switzerland) · 2023Review
- Transcriptome profiling of high and low somatic embryogenesis rate of oil palm (Frontiers in plant science · 2023Article
- Improving Coconut Using Modern Breeding Technologies: Challenges and Opportunities.Plants (Basel, Switzerland) · 2022Review
- Weighted Gene Correlation Network Analysis (WGCNA) ofInternational journal of genomics · 2022Article
- Cloning Coconut via Somatic Embryogenesis: A Review of the Current Status and Future Prospects.Plants (Basel, Switzerland) · 2021Review
- Transcriptomes analysis reveals novel insight into the molecular mechanisms of somatic embryogenesis in Hevea brasiliensis.BMC genomics · 2021Article
- Dataset of transcriptome assembly of date palm embryogenic calli and functional annotation.Data in brief · 2020Article
- De novo assembly and Transcriptome characterization of an endemic species of Vietnam, Panax vietnamensis Ha et Grushv., including the development of EST-SSR markers for population genetics.BMC plant biology · 2020Article
- Dynamic changes in the expression pattern of miRNAs and associated target genes during coconut somatic embryogenesis.Planta · 2020Article
- Global scale transcriptome analysis reveals differentially expressed genes involve in early somatic embryogenesis in Dimocarpus longan Lour.BMC genomics · 2020Article
- Article
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- Addition of ionophore A23187 increases the efficiency of3 Biotech · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Production and supply of quality planting material is significant to coconut cultivation but is one of the major constraints in coconut productivity. Rapid multiplication of coconut through in vitro techniques, therefore, is of paramount importance. Although somatic embryogenesis in coconut is a promising technique that will allow for the mass production of high quality palms, coconut is highly recalcitrant to in vitro culture. In order to overcome the bottlenecks in coconut somatic embryogenesis and to develop a repeatable protocol, it is imperative to understand, identify, and characterize molecular events involved in coconut somatic embryogenesis pathway. Transcriptome analysis (RNA-Seq) of coconut embryogenic calli, derived from plumular explants of West Coast Tall cultivar, was undertaken on an Illumina HiSeq 2000 platform. After de novo transcriptome assembly and functional annotation, we have obtained 40,367 transcripts which showed significant BLASTx matches with similarity greater than 40 % and E value of ≤10(-5). Fourteen genes known to be involved in somatic embryogenesis were identified. Quantitative real-time PCR (qRT-PCR) analyses of these 14 genes were carried in six developmental stages. The result showed that CLV was upregulated in the initial stage of callogenesis. Transcripts GLP, GST, PKL, WUS, and WRKY were expressed more in somatic embryo stage. The expression of SERK, MAPK, AP2, SAUR, ECP, AGP, LEA, and ANT were higher in the embryogenic callus stage compared to initial culture and somatic embryo stages. This study provides the first insights into the gene expression patterns during somatic embryogenesis in coconut.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.