ArticleApplied and environmental microbiology2015
A Continuous Culture System for Assessing Microbial Activities in the Piezosphere.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 20 citations in OpenAlex.
- Virulent strains of Pseudomonas aeruginosa are more disruptive during invasion of a microbial model community.FEMS microbiology letters · 2026Article
- High-pressure continuous culturing: life at the extreme.Applied and environmental microbiology · 2025Review
- Microbes from ambient-pressure analogues offer insights into possible life in Europa's high-pressure subsurface ocean.Frontiers in microbiology · 2025Article
- Exploring the impact of flow dynamics on corrosive biofilms under simulated deep-sea high-pressure conditions using bio-electrochemostasis.Frontiers in microbiology · 2025Article
- Adaptations to high pressure of Nautilia sp. strain PV-1, a piezophilic Campylobacterium (aka Epsilonproteobacterium) isolated from a deep-sea hydrothermal vent.Environmental microbiology · 2022Article
- Characterizing the Piezosphere: The Effects of Decompression on Microbial Growth Dynamics.Frontiers in microbiology · 2022Article
- Salinity and hydraulic retention time induce membrane phospholipid acyl chain remodeling inFrontiers in microbiology · 2022Article
- Yeast grown in continuous culture systems can detect mutagens with improved sensitivity relative to the Ames test.PloS one · 2021Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Continuous culture under elevated pressures is an important technique for expanding the exploration of microbial growth and survival in extreme environments associated with the deep biosphere. Here we present a benchtop stirred continuous culture bioreactor capable of withstanding temperatures ranging from 25 to 120°C and pressures as high as 69 MPa. The system is configured to allow the employment of media enriched in dissolved gases, under oxic or anoxic conditions, while permitting periodic sampling of the incubated organisms with minimal physical/chemical disturbance inside the reactor. In a pilot experiment, the fermentative growth of the thermopiezophilic bacterium Marinitoga piezophila was investigated continuously for 382 h at 65°C and at pressures ranging from 0.1 to 40 MPa while the medium flow rate was varied from 2 to 0.025 ml/min. The enhanced growth observed at 30 and 40 MPa and 0.025 ml/min supports the pressure preferences of M. piezophila when grown fermentatively. This assay successfully demonstrates the capabilities of the bioreactor for continuous culturing at a variety of dilution rates, pressures, and temperatures. We anticipate that this technology will accelerate our understanding of the physiological and metabolic status of microorganisms under temperature, pressure, and energy regimes resembling those of the Earth's piezosphere.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.