ArticleApplied and environmental microbiology2015
Fine-Tuning of Photoautotrophic Protein Production by Combining Promoters and Neutral Sites in the Cyanobacterium Synechocystis sp. Strain PCC 6803.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- Heterologous Production of the Structurally Complex Diterpenoid Forskolin inBioengineering (Basel, Switzerland) · 2025Article
- A toolbox to engineer the highly productive cyanobacterium Synechococcus sp. PCC 11901.Plant physiology · 2024Article
- Promoting Heme and Phycocyanin Biosynthesis inMarine drugs · 2023Article
- The current situations and limitations of genetic engineering in cyanobacteria: a mini review.Molecular biology reports · 2023Review
- Current Metabolic Engineering Strategies for Photosynthetic Bioproduction in Cyanobacteria.Microorganisms · 2023Review
- The Molecular Toolset and Techniques Required to Build Cyanobacterial Cell Factories.Advances in biochemical engineering/biotechnology · 2023Article
- Putative Phenotypically Neutral Genomic Insertion Points in Prokaryotes.ACS synthetic biology · 2022Article
- Synthetic Biology Tool Development Advances Predictable Gene Expression in the Metabolically Versatile Soil BacteriumFrontiers in bioengineering and biotechnology · 2022Article
- Approaches in the photosynthetic production of sustainable fuels by cyanobacteria using tools of synthetic biology.World journal of microbiology & biotechnology · 2021Review
- Comparison of alternative integration sites in the chromosome and the native plasmids of the cyanobacterium Synechocystis sp. PCC 6803 in respect to expression efficiency and copy number.Microbial cell factories · 2021Article
- Heterologous production of cyanobacterial compounds.Journal of industrial microbiology & biotechnology · 2021Review
- Screening neutral sites for metabolic engineering of methylotrophic yeastSynthetic and systems biotechnology · 2021Article
- Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications.Genes · 2021Review
- A suicide enzyme catalyzes multiple reactions for biotin biosynthesis in cyanobacteria.Nature chemical biology · 2020Article
- Progress and challenges in engineering cyanobacteria as chassis for light-driven biotechnology.Microbial biotechnology · 2020Article
- The Synthetic Biology Toolkit for Photosynthetic Microorganisms.Plant physiology · 2019Review
- State-of-the-Art Genetic Modalities to Engineer Cyanobacteria for Sustainable Biosynthesis of Biofuel and Fine-Chemicals to Meet Bio-Economy Challenges.Life (Basel, Switzerland) · 2019Review
- CyanoGate: A Modular Cloning Suite for Engineering Cyanobacteria Based on the Plant MoClo Syntax.Plant physiology · 2019Article
- Characterization of cyanobacterial cells synthesizing 10-methyl stearic acid.Photosynthesis research · 2019Article
- CRISPR-Cas9/Cas12a biotechnology and application in bacteria.Synthetic and systems biotechnology · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Cyanobacteria are photosynthetic cell factories that use solar energy to convert CO2 into useful products. Despite this attractive feature, the development of tools for engineering cyanobacterial chassis has lagged behind that for heterotrophs such as Escherichia coli or Saccharomyces cerevisiae. Heterologous genes in cyanobacteria are often integrated at presumptively "neutral" chromosomal sites, with unknown effects. We used transcriptome sequencing (RNA-seq) data for the model cyanobacterium Synechocystis sp. strain PCC 6803 to identify neutral sites from which no transcripts are expressed. We characterized the two largest such sites on the chromosome, a site on an endogenous plasmid, and a shuttle vector by integrating an enhanced yellow fluorescent protein (EYFP) expression cassette expressed from either the Pcpc560 or the Ptrc1O promoter into each locus. Expression from the endogenous plasmid was as much as 14-fold higher than that from the chromosome, with intermediate expression from the shuttle vector. The expression characteristics of each locus correlated predictably with the promoters used. These findings provide novel, characterized tools for synthetic biology and metabolic engineering in cyanobacteria.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.