ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2015
Diagnostics-in-a-Suitcase: Development of a portable and rapid assay for the detection of the emerging avian influenza A (H7N9) virus.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 98 citations in OpenAlex.
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- Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms.Biosensors · 2024Review
- A multiplex recombinase polymerase amplification assay combined with CRISPR/Cas12a for the detection of respiratory syncytial virus and respiratory adenovirus.The Journal of international medical research · 2024Article
- Rapid detection of avian influenza virus based on CRISPR-Cas12a.Virology journal · 2023Article
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- Evaluation of Recombinase Polymerase Amplification assay for monitoring parasite load in patients with kala-azar and post kala-azar dermal leishmaniasis.PLoS neglected tropical diseases · 2023Article
- Emerging Landscape of SARS-CoV-2 Variants and Detection Technologies.Molecular diagnosis & therapy · 2023Review
- Rapid detection of human coronavirus NL63 by isothermal reverse transcription recombinase polymerase amplification.Journal of clinical virology plus · 2022Article
- Reverse transcription recombinase-aided amplification assay for H5 subtype avian influenza virus.Virology journal · 2022Article
- Point-of-Care Diagnostics for Farm Animal Diseases: From Biosensors to Integrated Lab-on-Chip Devices.Biosensors · 2022Review
- Evaluation of recombinase-based isothermal amplification assays for point-of-need detection of SARS-CoV-2 in resource-limited settings.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2022Article
- Testing of NKA expression by mobile real time PCR is an efficient indicator of smoltification status of farmed Atlantic salmon.Aquaculture (Amsterdam, Netherlands) · 2021Article
- Molecular Detection of Feline Coronavirus Based on Recombinase Polymerase Amplification Assay.Pathogens (Basel, Switzerland) · 2021Article
- Article
- Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of Avian Influenza Virus H9N2 HA Gene.Veterinary sciences · 2021Article
- Ending the Neglect of Treatable Bacterial Zoonoses Responsible for Non-Malaria Fevers.The Yale journal of biology and medicine · 2021Review
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn developing countries, equipment necessary for diagnosis is only available in few central laboratories, which are less accessible and of limited capacity to test large numbers of incoming samples. Moreover, the transport conditions of samples are inadequate, therefore leading to unreliable results.
objectivesThe development of a rapid, inexpensive, and simple test would allow mobile detection of viruses. STUDY
designA suitcase laboratory "Diagnostics-in-a-Suitcase" (56cm×45.5cm×26.5cm) containing all reagents and devices necessary for performing a reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed. As an example, two RT-RPA assays were established for the detection of hemagglutinin (H) and neuraminidase (N) genes of the novel avian influenza (H7N9) virus.
resultsThe sensitivities of the H7 and the N9 RT-RPA assays were 10 and 100 RNA molecules, respectively. The assays were performed at a single temperature (42°C). The results were obtained within 2-7min. The H7N9 RT-RPA assays did not show a cross-detection either of any other respiratory viruses affecting humans and/or birds or of the human or chicken genomes. All reagents were used, stored, and transported at ambient temperature, that is, cold chain independent. In addition, the Diagnostics-in-a-Suitcase was operated by a solar-powered battery.
conclusionsThe developed assay protocol and mobile setup performed well. Moreover, it can be easily implemented to perform diagnoses at airports, quarantine stations, or farms for rapid on-site viral nucleic acid detection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.