ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2015
Epitope recognition in the human-pig comparison model on fixed and embedded material.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Indirect Methods to Determine the Risk of Damage to the Health of Firefighters and Children Due to Exposure to Smoke Emission from Burning Wood/Coal in a Controlled Environment.International journal of environmental research and public health · 2023Article
- Modulation of the Cardiac Myocyte Action Potential by the Magnesium-Sensitive TRPM6 and TRPM7-like Current.International journal of molecular sciences · 2021Article
- Accelerated instability testing reveals quantitative mass spectrometry overcomes specimen storage limitations associated with PD-L1 immunohistochemistry.Laboratory investigation; a journal of technical methods and pathology · 2020Article
- Synthetic Antigen Gels as Practical Controls for Standardized and Quantitative Immunohistochemistry.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2019Article
- Canine CD117-Specific Antibodies with Diverse Binding Properties Isolated from a Phage Display Library Using Cell-Based Biopanning.Antibodies (Basel, Switzerland) · 2019Article
- Multiplex Staining by Sequential Immunostaining and Antibody Removal on Routine Tissue Sections.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2017Article
- Antigen Masking During Fixation and Embedding, Dissected.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2017Article
- Optic nerve injury upregulates retinoic acid signaling in the adult frog visual system.Journal of chemical neuroanatomy · 2016Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The conditions and the specificity by which an antibody binds to its target protein in routinely fixed and embedded tissues are unknown. Direct methods, such as staining in a knock-out animal or in vitro peptide scanning of the epitope, are costly and impractical. We aimed to elucidate antibody specificity and binding conditions using tissue staining and public genomic and immunological databases by comparing human and pig-the farmed mammal evolutionarily closest to humans besides apes. We used a database of 146 anti-human antibodies and found that antibodies tolerate partially conserved amino acid substitutions but not changes in target accessibility, as defined by epitope prediction algorithms. Some epitopes are sensitive to fixation and embedding in a species-specific fashion. We also find that half of the antibodies stain porcine tissue epitopes that have 60% to 100% similarity to human tissue at the amino acid sequence level. The reason why the remaining antibodies fail to stain the tissues remains elusive. Because of its similarity with the human, pig tissue offers a convenient tissue for quality control in immunohistochemistry, within and across laboratories, and an interesting model to investigate antibody specificity.
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