ArticleBMC genomics2015
Common and specific downstream signaling targets controlled by Tlr2 and Tlr5 innate immune signaling in zebrafish.
Article in BMC genomics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Toll-like receptors in fish: evolution, structure, immune signaling, and translational prospects for adjuvant-based vaccine design in aquaculture.Frontiers in immunology · 2026Review
- Synergistic ROS Reduction Through the Co-Inhibition of BRAF and p38 MAPK Ameliorates Senescence.Antioxidants (Basel, Switzerland) · 2024Article
- Unveiling the multifaceted role of toll-like receptors in immunity of aquatic animals: pioneering strategies for disease management.Frontiers in immunology · 2024Review
- The Spike protein of SARS-CoV-2 signals via Tlr2 in zebrafish.Developmental and comparative immunology · 2023Article
- Article
- Inflammasomes in Teleosts: Structures and Mechanisms That Induce Pyroptosis during Bacterial Infection.International journal of molecular sciences · 2021Review
- Article
- A Novel Function of TLR2 and MyD88 in the Regulation of Leukocyte Cell Migration Behavior During Wounding in Zebrafish Larvae.Frontiers in cell and developmental biology · 2021Article
- Modeling Virus-Induced Inflammation in Zebrafish: A Balance Between Infection Control and Excessive Inflammation.Frontiers in immunology · 2021Review
- Article
- Intestinal microbiome adjusts the innate immune setpoint during colonization through negative regulation of MyD88.Nature communications · 2018Article
- Duplicated TLR5 of zebrafish functions as a heterodimeric receptor.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
- Disruption of Trim9 function abrogates macrophage motility in vivo.Journal of leukocyte biology · 2017Article
- Pattern recognition receptors in zebrafish provide functional and evolutionary insight into innate immune signaling pathways.Cellular & molecular immunology · 2017Review
- Leptospiral outer membrane protein LipL32 induces inflammation and kidney injury in zebrafish larvae.Scientific reports · 2016Article
- Fungal Innate Immunity Induced by Bacterial Microbe-Associated Molecular Patterns (MAMPs).G3 (Bethesda, Md.) · 2016Article
- Differential analyses for RNA-seq: transcript-level estimates improve gene-level inferences.F1000Research · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough the responses to many pathogen associated molecular patterns (PAMPs) in cell cultures and extracted organs are well characterized, there is little known of transcriptome responses to PAMPs in whole organisms. To characterize this in detail, we have performed RNAseq analysis of responses of zebrafish embryos to injection of PAMPs in the caudal vein at one hour after exposure. We have compared two ligands that in mammals have been shown to specifically activate the TLR2 and TLR5 receptors: Pam3CSK4 and flagellin, respectively.
resultsWe identified a group of 80 common genes that respond with high stringency selection to stimulations with both PAMPs, which included several well-known immune marker genes such as il1b and tnfa. Surprisingly, we also identified sets of 48 and 42 genes that specifically respond to either Pam3CSK4 or flagellin, respectively, after a comparative filtering approach. Remarkably, in the Pam3CSK4 specific set, there was a set of transcription factors with more than 2 fold-change, as confirmed by qPCR analyses, including cebpb, fosb, nr4a1 and egr3. We also showed that the regulation of the Pam3CSK4 and flagellin specifically responding sets is inhibited by knockdown of tlr2 or tlr5, respectively.
conclusionsOur studies show that Pam3CSK4 and flagellin can stimulate the Tlr2 and Tlr5 signaling pathways leading to common and specific responses in the zebrafish embryo system.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.