ArticleJournal of experimental botany2015
Methylation mediated by an anthocyanin, O-methyltransferase, is involved in purple flower coloration in Paeonia.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 113 citations in OpenAlex.
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- Somatic embryogenesis and genetic transformation in peony: challenges and applications.Frontiers in plant science · 2026Review
- FaTRAB1, a bZIP transcription factor, enhances anthocyanin biosynthesis in strawberry leaves via tissue-specific regulation.PLoS genetics · 2025Article
- Advancing ornamental plant breeding through genomic technologies: opportunities, challenges, and future directions.Functional & integrative genomics · 2025Review
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- High-quality genome of black wolfberry (Horticulture research · 2025Article
- Interaction of PsMYB4 with PsEGL3 inhibits anthocyanin biosynthesis in tree peony yellow flowers.Frontiers in plant science · 2025Article
- Methylation Modification in Ornamental Plants: Impact on Floral Aroma and Color.International journal of molecular sciences · 2024Review
- Genomic and transcriptomic studies on flavonoid biosynthesis in Lagerstroemia indica.BMC plant biology · 2024Article
- Flower morphology, flower color, flowering and floral fragrance inFrontiers in plant science · 2024Review
- Genome-Wide Identification and Characterization of thePlants (Basel, Switzerland) · 2023Article
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- A multifunctional true caffeoyl coenzyme A O-methyltransferase enzyme participates in the biosynthesis of polymethoxylated flavones in citrus.Plant physiology · 2023Article
- Regioselective stilbene O-methylations in Saccharinae grasses.Nature communications · 2023Article
- The combination of DNA methylation and positive regulation of anthocyanin biosynthesis by MYB and bHLH transcription factors contributes to the petal blotch formation in Xibei tree peony.Horticulture research · 2023Article
- Tree peony PsMYB44 negatively regulates petal blotch distribution by inhibiting dihydroflavonol-4-reductase gene expression.Annals of botany · 2023Article
- Article
- Metabolic profile and transcriptome reveal the mystery of petal blotch formation in rose.BMC plant biology · 2023Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Anthocyanins are major pigments in plants. Methylation plays a role in the diversity and stability of anthocyanins. However, the contribution of anthocyanin methylation to flower coloration is still unclear. We identified two homologous anthocyanin O-methyltransferase (AOMT) genes from purple-flowered (PsAOMT) and red-flowered (PtAOMT) Paeonia plants, and we performed functional analyses of the two genes in vitro and in vivo. The critical amino acids for AOMT catalytic activity were studied by site-directed mutagenesis. We showed that the recombinant proteins, PsAOMT and PtAOMT, had identical substrate preferences towards anthocyanins. The methylation activity of PsAOMT was 60 times higher than that of PtAOMT in vitro. Interestingly, this vast difference in catalytic activity appeared to result from a single amino acid residue substitution at position 87 (arginine to leucine). There were significant differences between the 35S::PsAOMT transgenic tobacco and control flowers in relation to their chromatic parameters, which further confirmed the function of PsAOMT in vivo. The expression levels of the two homologous AOMT genes were consistent with anthocyanin accumulation in petals. We conclude that AOMTs are responsible for the methylation of cyanidin glycosides in Paeonia plants and play an important role in purple coloration in Paeonia spp.
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