ArticlePloS one2015
Differential Contribution of TRPA1, TRPV4 and TRPM8 to Colonic Nociception in Mice.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 58 citations in OpenAlex.
- Transient Receptor Potential Melastatin 8 Plays a Key Role in Dextran Sulfate Sodium-Induced Murine Colitis via Regulating Extrinsic Sensory Neurons and Enteric Macrophage Secretory Activities.Neurogastroenterology and motility · 2026Article
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- STIM1 functionally couples to transient receptor potential ankyrin 1 contributing to nociception.Pain · 2025Article
- TRPM8 inhibits substance P release from primary sensory neurons via PKA/GSK-3beta to protect colonic epithelium in colitis.Cell death & disease · 2024Article
- Article
- Article
- The role of TRPV4 in the regulation of retinal ganglion cells apoptosis in rat and mouse.Heliyon · 2023Article
- Intestinal distension orchestrates neuronal activity in the enteric nervous system of adult mice.The Journal of physiology · 2023Article
- Intrarectal Capsazepine Administration Modulates Colonic Mucosal Health in Mice.International journal of molecular sciences · 2022Article
- Nobel somatosensations and pain.Pflugers Archiv : European journal of physiology · 2022Review
- PRDM16, LRP1 and TRPM8 genetic polymorphisms are risk factor for Pakistani migraine patients.Saudi journal of biological sciences · 2021Article
- Role of TRPV4-P2X7 Pathway in Neuropathic Pain in Rats with Chronic Compression of the Dorsal Root Ganglion.Neurochemical research · 2021Article
- Article
- Role of TRPA1 in Tissue Damage and Kidney Disease.International journal of molecular sciences · 2021Review
- TRPM8 Channel Activation Reduces the Spontaneous Contractions in Human Distal Colon.International journal of molecular sciences · 2020Article
- Transient Receptor Potential Channels and Inflammatory Bowel Disease.Frontiers in immunology · 2020Review
- Involvement of Neural Transient Receptor Potential Channels in Peripheral Inflammation.Frontiers in immunology · 2020Review
- Transient receptor potential ankyrin 1 promoter methylation and peripheral pain sensitivity in Crohn's disease.Clinical epigenetics · 2019Article
- Characterization of New TRPM8 Modulators in Pain Perception.International journal of molecular sciences · 2019Article
- Intrathecal TRPM8 blocking attenuates cold hyperalgesia via PKC and NF-κB signaling in the dorsal root ganglion of rats with neuropathic pain.Journal of pain research · 2019Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVarious transient receptor potential (TRP) channels in sensory neurons contribute to the transduction of mechanical stimuli in the colon. Recently, even the cold-sensing menthol receptor TRPM(melastatin)8 was suggested to be involved in murine colonic mechano-nociception.
methodsTo analyze the roles of TRPM8, TRPA1 and TRPV4 in distension-induced colonic nociception and pain, TRP-deficient mice and selective pharmacological blockers in wild-type mice (WT) were used. Visceromotor responses (VMR) to colorectal distension (CRD) in vivo were recorded and distension/pressure-induced CGRP release from the isolated murine colon ex vivo was measured by EIA.
resultsDistension-induced colonic CGRP release was markedly reduced in TRPA1-/- and TRPV4-/- mice at 90/150 mmHg compared to WT. In TRPM8-deficient mice the reduction was only distinct at 150 mmHg. Exposure to selective pharmacological antagonists (HC030031, 100 μM; RN1734, 10 μM; AMTB, 10 μM) showed corresponding effects. The unselective TRP blocker ruthenium red (RR, 10 μM) was as efficient in inhibiting distension-induced CGRP release as the unselective antagonists of mechanogated DEG/ENaC (amiloride, 100 μM) and stretch-activated channels (gadolinium, 50 μM). VMR to CRD revealed prominent deficits over the whole pressure range (up to 90 mmHg) in TRPA1-/- and TRPV4-/- but not TRPM8-/- mice; the drug effects of the TRP antagonists were again highly consistent with the results from mice lacking the respective TRP receptor gene.
conclusionsTRPA1 and TRPV4 mediate colonic distension pain and CGRP release and appear to govern a wide and congruent dynamic range of distensions. The role of TRPM8 seems to be confined to signaling extreme noxious distension, at least in the healthy colon.
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