ArticleRadiation research2015
A Mouse Ear Model for Bystander Studies Induced by Microbeam Irradiation.
Article in Radiation research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 19 citations in OpenAlex.
- Bystander Effects in Osteoblasts and Osteoclasts: Comparison Between X- and Proton-Irradiation.International journal of particle therapy · 2025Article
- The intercellular communications mediating radiation-induced bystander effects and their relevance to environmental, occupational, and therapeutic exposures.International journal of radiation biology · 2023Review
- Radiation-Induced Bystander Effect and Cytoplasmic Irradiation Studies with Microbeams.Biology · 2022Review
- Regenerative Drug Discovery Using Ear Pinna Punch Wound Model in Mice.Pharmaceuticals (Basel, Switzerland) · 2022Article
- The Radiobiology of Radiopharmaceuticals.Seminars in radiation oncology · 2021Review
- Prevention of radiation-induced bystander effects by agents that inactivate cell-free chromatin released from irradiated dying cells.Cell death & disease · 2018Article
- Reflections on Basic Science Studies Involving Low Doses of Ionizing Radiation.Health physics · 2018Article
- Epithelial-mesenchymal transition in non-targeted lung tissues of Kunming mice exposed to X-rays is suppressed by celecoxib.Journal of radiation research · 2018Article
- Focus small to find big - the microbeam story.International journal of radiation biology · 2018Review
- Microbeam radiation therapy - grid therapy and beyond: a clinical perspective.The British journal of radiology · 2017Review
- 50 Years of the Radiological Research Accelerator Facility (RARAF).Radiation research · 2017Article
- Molecular Understanding of Growth Inhibitory Effect from Irradiated to Bystander Tumor Cells in Mouse Fibrosarcoma Tumor Model.PloS one · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
Radiation-induced bystander effects have been observed in vitro and in cell and tissue culture models, however, there are few reported studies showing these effects in vivo. To our knowledge, this is the first reported study on bystander effects induced by microbeam irradiation in an intact living mammal. The mouse ear was used to investigate radiation-induced bystander effects in keratinocytes, utilizing a 3 MeV proton microbeam (LET 13.1 keV/μm) with a range in skin of about 135 μm. Using a custom-designed holder, the ear of an anesthetized C57BL/6J mouse was flattened by gentle suction and placed over the microbeam port to irradiate cells along a 35 μm wide, 6 mm long path. Immunohistochemical analysis of γ-H2AX foci formation in tissue sections revealed, compared to control tissue, proton-induced γ-H2AX foci formation in one of the two epidermal layers of the mouse ear. Strikingly, a higher number of cells than expected showed foci from direct irradiation effects. Although the proton-irradiated line was ~35 μm wide, the average width spanned by γ-H2AX-positive cells exceeded 150 μm. Cells adjacent to or in the epidermal layer opposite the γ-H2AX-positive region did not exhibit foci. These findings validate this mammalian model as a viable system for investigating radiation-induced bystander effects in an intact living organism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.